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Published online on October 30, 2001, 10.1073/pnas.231494498
PNAS | November 6, 2001 | vol. 98 | no. 23 | 13207-13212


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Genetics
 Retrotransposition of a yeast group II intron occurs by reverse splicing directly into ectopic DNA sites

Lorna Dickson*,dagger ,Dagger , Hon-Ren Huang*,dagger , Lu Liu*, Manabu Matsuura§,, Alan M. Lambowitz§, and Philip S. Perlman*,||

* Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148; and § Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, and Section of Molecular Genetics and Microbiology, University of Texas, Austin, TX 78712

Communicated by Marlene Belfort, New York State Department of Health, Albany, NY, September 19, 2001 (received for review July 23, 2001)

Group II introns, the presumed ancestors of nuclear pre-mRNA introns, are site-specific retroelements. In addition to "homing" to unoccupied sites in intronless alleles, group II introns transpose at low frequency to ectopic sites that resemble the normal homing site. Two general mechanisms have been proposed for group II intron transposition, one involving reverse splicing of the intron RNA directly into an ectopic DNA site, and the other involving reverse splicing into a site in RNA followed by reverse transcription and integration of the resulting cDNA by homologous recombination. Here, by using an "inverted-site" strategy, we show that the yeast mtDNA group II intron aI1 retrotransposes by reverse splicing directly into an ectopic DNA site. This same mechanism could account for other previously described ectopic transposition events in fungi and bacteria and may have contributed to the dispersal of group II introns into different genes.

dagger L.D. and H.-R.H. contributed equally to this work.

Dagger Present address: Pacific Northwest Research Institute, 720 Broadway, Seattle, WA 98122.

Present address: National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan, 305-8562.

|| To whom reprint requests should be addressed. E-mail: philip.perlman{at}utsouthwestern.edu.

www.pnas.org/cgi/doi/10.1073/pnas.231494498
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