Mapping the early steps in the pH-induced conformational conversion of the prion protein
- *Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195-7610; and Departments of †Pathology (Neuropathology), and ‡Molecular and Cellular Pharmacology, University of California, San Francisco, CA 94143
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Edited by Alan Fersht, University of Cambridge, Cambridge, United Kingdom, and approved January 4, 2001 (received for review November 22, 2000)
Abstract
Under certain conditions, the prion protein (PrP) undergoes a conformational change from the normal cellular isoform, PrPC, to PrPSc, an infectious isoform capable of causing neurodegenerative diseases in many mammals. Conversion can be triggered by low pH, and in vivo this appears to take place in an endocytic pathway and/or caveolae-like domains. It has thus far been impossible to characterize the conformational change at high resolution by experimental methods. Therefore, to investigate the effect of acidic pH on PrP conformation, we have performed 10-ns molecular dynamics simulations of PrPC in water at neutral and low pH. The core of the protein is well maintained at neutral pH. At low pH, however, the protein is more dynamic, and the sheet-like structure increases both by lengthening of the native β-sheet and by addition of a portion of the N terminus to widen the sheet by another two strands. The side chain of Met-129, a polymorphic codon in humans associated with variant Creutzfeldt–Jakob disease, pulls the N terminus into the sheet. Neutralization of Asp-178 at low pH removes interactions that inhibit conversion, which is consistent with the Asp-178–Asn mutation causing human prion diseases.
Footnotes
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↵ § To whom reprint requests should be addressed. E-mail: daggett{at}u.washington.edu.
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This paper was submitted directly (Track II) to the PNAS office.
- Abbreviations:
- PrP,
- prior protein;
- HA,
- helix A;
- S1,
- strand 1;
- S2,
- strand 2
- Copyright © 2001, The National Academy of Sciences
