Topical colchicine selection of keratinocytes transduced with the multidrug resistance gene ( MDR1 ) can sustain and enhance transgene expression in vivo

doi:10.1073/pnas.192247899

Topical colchicine selection of keratinocytes transduced with the multidrug resistance gene (MDR1) can sustain and enhance transgene expression in vivo

^*Dermatology Branch, Building 10/Room 12N260, National Cancer Institute, National Institutes of Health, 10 Center Drive, MSC 1908, Bethesda, MD 20892-1908; ^§Laboratory of Cell Biology, Building 37/Room 1A09, National Cancer Institute, National Institutes of Health, 37 Convent Drive, MSC 4254, Bethesda, MD 20892-4254; and ^‡Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York, Stony Brook, NY 11794-8702

Edited by Ira Pastan, National Institutes of Health, Bethesda, MD, and approved June 27, 2002 (received for review April 25, 2002)

Abstract

For skin gene therapy, achieving prolonged high-level gene expression in a significant percentage of keratinocytes (KC) is difficult because we cannot selectively target KC stem cells. We now demonstrate that topical colchicine treatment can be used to select, in vivo, KC progenitor cells transduced with the multidrug resistance gene (MDR1). When human skin equivalents containing MDR1-transduced KC were grafted onto immunocompromised mice, topical colchicine treatments significantly increased (7-fold) the percentage of KC expressing MDR1, compared to vehicle-treated controls, for up to 24 wk. Topical colchicine treatment also significantly enhanced the amount of MDR1 protein expressed in individual KC. Furthermore, quantitative real-time PCR analysis of MDR1 transgene copy number demonstrates that topical colchicine treatment selects and enriches for KC progenitor cells in the skin that contain and express MDR1. For clinical skin gene therapy applications, this in vivo selection approach promises to enhance both the duration and expression level of a desired therapeutic gene in KC, by linking its expression to the MDR1 selectable marker gene.

Footnotes

↵ † Present address: Dermatology Clinic, Ludwig Maximilians University of Munich, 80337 Munich, Germany.
↵ ¶ To whom reprint requests should be addressed. E-mail: jonvogel{at}mail.nih.gov.
This paper was submitted directly (Track II) to the PNAS office.
Abbreviations:

KC,

keratinocytes;

hKC,

human KC;

FB,

fibroblasts;

FACS,

fluorescence-activated cell sorter