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PNAS | February 5, 2002 | vol. 99 | no. 3 | 1718-1723
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Plant Biology
 A gene encoding a protein modified by the phytohormone indoleacetic acid

Alexander Walz*,dagger ,Dagger ,§, Seijin Park*,Dagger ,§, Janet P. SlovinDagger , Jutta Ludwig-Müllerdagger , Yoshie S. Momonoki, and Jerry D. Cohen*,||

* Department of Horticultural Science, University of Minnesota, Saint Paul, MN 55108; dagger  Institut für Botanik, Technische Universität Dresden, 01062 Dresden, Germany; Dagger  Fruit Laboratory, United States Department of Agriculture/Agricultural Research Service, Beltsville, MD 20705; and  Faculty of Bioindustry, Tokyo University of Agriculture, 099-2493 Hokkaido, Japan

Edited by Bernard Phinney, University of California, Los Angeles, CA, and approved December 10, 2001 (received for review August 23, 2001)

We show that the expression of an indole-3-acetic acid (IAA)-modified protein from bean seed, IAP1, is correlated to the developmental period of rapid growth during seed development. Moreover, this protein undergoes rapid degradation during germination. The gene for IAP1, the most abundant protein covalently modified by IAA (iap1, GenBank accession no. AF293023) was isolated and cloned from bush bean (Phaseolus vulgaris) seeds. The 957-bp sequence encodes a 35-kDa polypeptide. IAA-modified proteins represent a distinct class of conjugated phytohormones and appear in bean to be the major form of auxin in seeds. IAA proteins also are found at other stages of development in bean plants. Our immunological and analytical data suggest that auxin modification of a small class of proteins may be a feature common to many plants.

§ A.W. and S.P. contributed equally to this work.

|| To whom reprint requests should be addressed at: University of Minnesota, 305 Alderman Hall, 1970 Folwell Avenue, Saint Paul, MN 55108. E-mail: cohen047{at}tc.umn.edu.

www.pnas.org/cgi/doi/10.1073/pnas.032450399
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