Track the topics, authors, and articles important to you. Sign up for PNAS Online eTocs
Link: Info for AuthorsLink: Editorial BoardLink: AboutLink: SubscribeLink: AdvertiseLink: ContactLink: Sitemap Link: PNAS Home
Proceedings of the National Academy of Sciences
Link: Current Issue "" Link: Archives "" Link: Online Submission ""  Link: Advanced Search

Published online on April 23, 2002, 10.1073/pnas.092131899
PNAS | April 30, 2002 | vol. 99 | no. 9 | 6053-6058


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a colleague
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My File Cabinet
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (72)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, Z.
Right arrow Articles by Rosen, B. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, Z.
Right arrow Articles by Rosen, B. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg  
What's this?

 Previous Article  | Table of Contents |  Next Article 

Cell Biology
 Arsenite transport by mammalian aquaglyceroporins AQP7 and AQP9

(Fps1p|GlpF|acute promyelocytic leukemia)

Zijuan Liu*, Jian Shen*, Jennifer M. Carbreydagger , Rita Mukhopadhyay*, Peter Agredagger , and Barry P. Rosen*,Dagger

* Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201; and dagger  Departments of Biological Chemistry and Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205

Contributed by Peter Agre, March 7, 2002

Much is known about the transport of arsenite and antimonite into microbes, but the identities of mammalian transport proteins are unknown. The Saccharomyces cerevisiae FPS1 gene encodes a membrane protein homologous to the bacterial aquaglyceroporin GlpF and to mammalian aquaglyceroporins AQP7 and AQP9. Fps1p mediates glycerol uptake and glycerol efflux in response to hypoosmotic shock. Fps1p has been shown to facilitate uptake of the metalloids arsenite and antimonite, and the Escherichia coli homolog, GlpF, facilitates the uptake and sensitivity to metalloid salts. In this study, the ability of mammalian aquaglyceroporins AQP7 and AQP9 to substitute for the yeast Fps1p was examined. The fps1Delta strain of S. cerevisiae exhibits increased tolerance to arsenite and antimonite compared to a wild-type strain. Introduction of a plasmid containing AQP9 reverses the metalloid tolerance of the deletion strain. AQP7 was not expressed in yeast. The fps1Delta cells exhibit reduced transport of 73As(III) or 125Sb(III), but uptake is enhanced by expression of AQP9. Xenopus laevis oocytes microinjected with either AQP7 or AQP9 cRNA exhibited increased transport of 73As(III). These results suggest that AQP9 and AQP7 may be a major routes of arsenite uptake into mammalian cells, an observation potentially of large importance for understanding the action of arsenite as a human toxin and carcinogen, as well as its efficacy as a chemotherapeutic agent for acute promyelocytic leukemia.

Dagger To whom reprint requests should be addressed. E-mail: brosen{at}med.wayne.edu.

www.pnas.org/cgi/doi/10.1073/pnas.092131899
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg    What's this?


This article has been cited by other articles in HighWire Press-hosted journals:


Home page
 J. Biol. Chem.Home page
S. Sundaram, B. Rathinasabapathi, L. Q. Ma, and B. P. Rosen
An Arsenate-activated Glutaredoxin from the Arsenic Hyperaccumulator Fern Pteris vittata L. Regulates Intracellular Arsenite
J. Biol. Chem., March 7, 2008; 283(10): 6095 - 6101.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
N. L. Uzcategui, D. Carmona-Gutierrez, V. Denninger, C. Schoenfeld, F. Lang, K. Figarella, and M. Duszenko
Antiproliferative Effect of Dihydroxyacetone on Trypanosoma brucei Bloodstream Forms: Cell Cycle Progression, Subcellular Alterations, and Cell Death
Antimicrob. Agents Chemother., November 1, 2007; 51(11): 3960 - 3968.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Matsumura, B. H.-J. Chang, M. Fujimiya, W. Chen, R. N. Kulkarni, Y. Eguchi, H. Kimura, H. Kojima, and L. Chan
Aquaporin 7 Is a {beta}-Cell Protein and Regulator of Intraislet Glycerol Content and Glycerol Kinase Activity, {beta}-Cell Mass, and Insulin Production and Secretion
Mol. Cell. Biol., September 1, 2007; 27(17): 6026 - 6037.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
Y. Wang and E. Tajkhorshid
Molecular Mechanisms of Conduction and Selectivity in Aquaporin Water Channels
J. Nutr., June 1, 2007; 137(6): 1509S - 1515S.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. T. Skowronski, J. Lebeck, A. Rojek, J. Praetorius, E.-M. Fuchtbauer, J. Frokiaer, and S. Nielsen
AQP7 is localized in capillaries of adipose tissue, cardiac and striated muscle: implications in glycerol metabolism
Am J Physiol Renal Physiol, March 1, 2007; 292(3): F956 - F965.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
Ashutosh, S. Sundar, and N. Goyal
Molecular mechanisms of antimony resistance in Leishmania
J. Med. Microbiol., February 1, 2007; 56(2): 143 - 153.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Thorsen, Y. Di, C. Tangemo, M. Morillas, D. Ahmadpour, C. Van der Does, A. Wagner, E. Johansson, J. Boman, F. Posas, et al.
The MAPK Hog1p Modulates Fps1p-dependent Arsenite Uptake and Tolerance in Yeast
Mol. Biol. Cell, October 1, 2006; 17(10): 4400 - 4410.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. R. Ellis, L. Gumaelius, E. Indriolo, I. J. Pickering, J. A. Banks, and D. E. Salt
A Novel Arsenate Reductase from the Arsenic Hyperaccumulating Fern Pteris vittata
Plant Physiology, August 1, 2006; 141(4): 1544 - 1554.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T.-C. Lee, I-C. Ho, W.-J. Lu, and J.-d. Huang
Enhanced Expression of Multidrug resistance-associated Protein 2 and Reduced Expression of Aquaglyceroporin 3 in an Arsenic-resistant Human Cell Line
J. Biol. Chem., July 7, 2006; 281(27): 18401 - 18407.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
H.-C. Yang, J. Cheng, T. M. Finan, B. P. Rosen, and H. Bhattacharjee
Novel Pathway for Arsenic Detoxification in the Legume Symbiont Sinorhizobium meliloti
J. Bacteriol., October 15, 2005; 187(20): 6991 - 6997.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. Amiry-Moghaddam, H. Lindland, S. Zelenin, B. A. Roberg, B. B. Gundersen, P. Petersen, E. Rinvik, I. A. Torgner, and O. P. Ottersen
Brain mitochondria contain aquaporin water channels: evidence for the expression of a short AQP9 isoform in the inner mitochondrial membrane
FASEB J, September 1, 2005; 19(11): 1459 - 1467.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
E. Dopp, L. M. Hartmann, U. von Recklinghausen, A. M. Florea, S. Rabieh, U. Zimmermann, B. Shokouhi, S. Yadav, A. V. Hirner, and A. W. Rettenmeier
Forced Uptake of Trivalent and Pentavalent Methylated and Inorganic Arsenic and Its Cyto-/genotoxicity in Fibroblasts and Hepatoma Cells
Toxicol. Sci., September 1, 2005; 87(1): 46 - 56.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. S. Verkman
More than just water channels: unexpected cellular roles of aquaporins
J. Cell Sci., August 1, 2005; 118(15): 3225 - 3232.
[Abstract] [Full Text] [PDF]

Home page
 Arch Intern MedHome page
W.-Y. Au, G. T. Cheung, T. W. Yuen, C. R. Kumana, and Y.-L. Kwong
Successful Treatment of Relapsed Acute Promyelocytic Leukemia in a Patient Receiving Continuous Ambulatory Peritoneal Dialysis With Oral Arsenic Trioxide
Arch Intern Med, May 9, 2005; 165(9): 1067 - 1068.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Karlgren, N. Pettersson, B. Nordlander, J. C. Mathai, J. L. Brodsky, M. L. Zeidel, R. M. Bill, and S. Hohmann
Conditional Osmotic Stress in Yeast: A SYSTEM TO STUDY TRANSPORT THROUGH AQUAGLYCEROPORINS AND OSMOSTRESS SIGNALING
J. Biol. Chem., February 25, 2005; 280(8): 7186 - 7193.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Maeda, T. Funahashi, T. Hibuse, A. Nagasawa, K. Kishida, H. Kuriyama, T. Nakamura, S. Kihara, I. Shimomura, and Y. Matsuzawa
Adaptation to fasting by glycerol transport through aquaporin 7 in adipose tissue
PNAS, December 21, 2004; 101(51): 17801 - 17806.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Li, O. P. Dhankher, L. Carreira, D. Lee, A. Chen, J. I. Schroeder, R. S. Balish, and R. B. Meagher
Overexpression of Phytochelatin Synthase in Arabidopsis Leads to Enhanced Arsenic Tolerance and Cadmium Hypersensitivity
Plant Cell Physiol., December 15, 2004; 45(12): 1787 - 1797.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhou, N. Messier, M. Ouellette, B. P. Rosen, and R. Mukhopadhyay
Leishmania major LmACR2 Is a Pentavalent Antimony Reductase That Confers Sensitivity to the Drug Pentostam
J. Biol. Chem., September 3, 2004; 279(36): 37445 - 37451.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. A. Hansen, F. Dacheux, S. Y. Man, J. Clulow, and R. C. Jones
Fluid Reabsorption by the Ductuli Efferentes Testis of the Rat Is Dependent on Both Sodium and Chlorine
Biol Reprod, August 1, 2004; 71(2): 410 - 416.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Gourbal, N. Sonuc, H. Bhattacharjee, D. Legare, S. Sundar, M. Ouellette, B. P. Rosen, and R. Mukhopadhyay
Drug Uptake and Modulation of Drug Resistance in Leishmania by an Aquaglyceroporin
J. Biol. Chem., July 23, 2004; 279(30): 31010 - 31017.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-L. Meng, Z. Liu, and B. P. Rosen
As(III) and Sb(III) Uptake by GlpF and Efflux by ArsB in Escherichia coli
J. Biol. Chem., April 30, 2004; 279(18): 18334 - 18341.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Liu, E. Boles, and B. P. Rosen
Arsenic Trioxide Uptake by Hexose Permeases in Saccharomyces cerevisiae
J. Biol. Chem., April 23, 2004; 279(17): 17312 - 17318.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
C. Brochu, J. Wang, G. Roy, N. Messier, X.-Y. Wang, N. G. Saravia, and M. Ouellette
Antimony Uptake Systems in the Protozoan Parasite Leishmania and Accumulation Differences in Antimony-Resistant Parasites
Antimicrob. Agents Chemother., October 1, 2003; 47(10): 3073 - 3079.
[Abstract] [Full Text] [PDF]