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Microbial diversity in the deep sea and the underexplored “rare biosphere”
- Mitchell L. Sogin*,†,
- Hilary G. Morrison*,
- Julie A. Huber*,
- David Mark Welch*,
- Susan M. Huse*,
- Phillip R. Neal*,
- Jesus M. Arrieta‡,§, and
- Gerhard J. Herndl‡
- *Josephine Bay Paul Center, Marine Biological Laboratory at Woods Hole, 7 MBL Street, Woods Hole, MA 02543; and
- ‡Royal Netherlands Institute for Sea Research, P.O. Box 59, 1790 AB, Den Burg, Texel, The Netherlands
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Communicated by M. S. Meselson, Harvard University, Cambridge, MA, June 20, 2006 (received for review May 5, 2006)
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Fig. 1.Rarefaction analysis for each sample based on best matches against the V6RefDB database. The frequency of observed best matches to V6RefDB (OTUs) for each site was used to calculate rarefaction curves with the program Analytic Rarefaction 1.3.
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Fig. 2.Rarefaction analysis for sample FS396 based on pairwise distance. Rarefaction is shown for OTUs that contain unique sequences and OTUs with differences that do not exceed 1%, 2%, 3%, 5%, or 10%. Rarefaction of the other seven samples showed curves with similar slopes.
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Fig. 3.Similarity of 454 sequence tags from FS396 to the V6RefDB database. “All tag distribution” plots the number of tag sequences for all samples versus the percentage difference from the best-matching sequence in V6RefDB. “Percent unique reads” from all samples shows the percentage difference between each distinct tag sequence and its best match in V6RefDB. “Percent total tags” plots the cumulative percentage of reads in all samples at or below a given percentage difference from best matches in V6RefDB.
Footnotes
- †To whom correspondence should be addressed. E-mail: sogin{at}mbl.edu
- © 2006 by The National Academy of Sciences of the USA
