Insight into the PrP C → PrP Sc conversion from the structures of antibody-bound ovine prion scrapie-susceptibility variants

Eghiaian et al. 10.1073/pnas.0400014101.

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Fig. 5. Western blot analysis of sheep PrPC and of proteinase K-treated PrPSc and PrPC used for the ELISA: proteinase K digestion of the brain homogenates as described in Materials and Methods. Lane 1, normal sheep brain homogenate; lane 2, proteinase K-digested infected sheep brain homogenate; lane 3, proteinase K-digested normal sheep brain.





Fig. 6. ELISA titration of PrPC and of proteinase K (PK)-treated PrPC and PrPSc from mouse (Mo), hamster (SHa), and macaque (Ma). PrPC samples are from brains of normal mouse, hamster, and macaque. PrPSc is produced by proteinase K treatment of homogenates of brains infected by scrapie for mouse and hamster and by bovine spongiform encephalopathy for macaque. The proteinase K-treated PrPC samples are used as a control for total digestion of PrPC. (Top) Mouse. (Middle) Macaque. (Bottom) Hamster.

This Article

  1. Published online before print July 6, 2004, doi: 10.1073/pnas.0400014101 PNAS July 13, 2004 vol. 101 no. 28 10254-10259
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