Electrostatically optimized Ras-binding Ral guanine dissociation stimulator mutants increase the rate of association by stabilizing the encounter complex

Kiel et al. 10.1073/pnas.0401160101.

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Fig. 6. Plot of the observed rate constant kobs against the Raf–Ras-binding domain (Raf-RBD) concentration for the binding of Ras·2′,3′-N-methylanthraniloyl-5′-guanosyl-b, g -imido-triphosphate to Raf-RBDWT. These measurements have been performed at 15°C in 15 mM Hepes buffer, pH 7.4/5 mM MgCl2/125 mM NaCl. The curve was fitted according to Eq. 1.

This Article

  1. Published online before print June 14, 2004, doi: 10.1073/pnas.0401160101 PNAS June 22, 2004 vol. 101 no. 25 9223-9228
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