High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase

Kim et al. 10.1073/pnas.0401722101.

Supporting Information

Files in this Data Supplement:

Fig. 6. Luminescence intensity induced by various steroid hormones and chemicals. The cotransfected cells were incubated, respectively, with 10^-6 M of 5a -dihydrotestosterone (DHT), testosterone (Tes), 19-nortestosterone (19nT), 17b -estradiol (E2), progesterone (Prog), vinclozolin (Vin), 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl)ethane (DDT), cyproterone acetate (CPA), flutamide (Flu), procymidone (Proc), forskolin, 12-myristate-13-acetate (PMA), and polychlorinated biphenyls (Aroclor 1254; PCB) (n = 3). Stimulation with DHT of the cells transfected only with pcDRc-AR was included as a background luminescence (DHT wo N-ter).

Fig. 7. In vivo optical imaging of mice carrying transiently transfected COS-7 cells. (A) In vivo optical charge-coupled device (CCD) imaging of androgen receptor (AR) translocation into the nucleus in living mice. The mice were imaged with coelenterazine 16 h after the s.c. implantation of COS-7 cells transiently transfected with pcRDn-NLS (site 2), pcDRc-AR (site 3), pcRDn-NLS and pcDRc-AR (site 4), and of mock transfected cells (site 1). The pseudocolor image of bioluminescence intensities was superimposed on photographic images of mice with a scale in photons/sec per cm². Each implanted site was defined as a region-of-interest (ROI). (B) The average of the photon count of each implanted site after the i.p. injection of coelenterazine (1.4 mg/kg body weight). The average values of photon counts for three mice were (1.41 ± 0.24) ´10⁴ (left front leg), (1.54 ± 0.31) ´10⁴ (right front leg), (4.07 ± 0.06) ´10⁴ (left hind leg), and (4.16 ± 0.37) ´10⁵ (right hind leg) (photons/sec per cm²). (C) Time course of light production from each site of the mouse (A) after i.p. injection of coelenterazine (2.8 mg/kg body weight).

• Early Edition
• Archives
• Online Submission

• Feature Articles

  (Expanded research articles of exceptional breadth)

• Commentaries

  (Expert commentary on leading research)

• Letters

  (Online-only letters to the editor)

• Inaugural Articles

  (Articles by recently elected NAS members)

• PNAS Profiles

  (Biographical profiles of Academy members)

• Sustainability Science

  (Interactions of human and environmental systems)

• Collected Papers

  (Editorials, Perspectives, Reviews, Colloquia, etc.)

• Special Features

  (Solicited articles on exceptional research topics)

• Sackler Colloquia

  (Scientific reports held under NAS auspices)