Dietary approach to attenuate oxidative stress, hypertension, and inflammation in the cardiovascular system

Wu et al. 10.1073/pnas.0402004101.

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Fig. 7. HPLC analyses of desulfoglucosinolates in broccoli sprouts. (A) The glucosinolates present in the broccoli sprouts that were air-dried include the following: glucoiberin (9.6 min), progoitrin (11.7 min), sinigrin (15.5 min), glucoraphanin (19.2 min), glucobrassicanapin (34.3 min), glucobrassican (43.1 min), and gluconasturtiin (51.6 min). There were several small unidentified peaks at 30.1 and 31.1 min, and the internal standard is represented by the 33.3-min peak. (B) No glucosinolates were detectable in broccoli sprouts that were frozen, thawed, and dried. The 33.3-min peak represents the internal standard o-nitrophenyl-D-galactopyranoside.

Fig. 8. A nitrosylated 45-kDa protein is present in SHRsp kidney tissue. (A) Lane 1 demonstrates representative immunoblots for the 45-kDa protein, whereas lane 2 represents b -actin levels in the same extracts from SHR-CT, SHR-Grn^–, and SHRsp-Grn⁺. Represented in this figure are samples from two animals in each group. (B) Graph that demonstrates quantification (y axis is in relative units) of the nitrosylated protein relative to b -actin in the three diet groups. *, P < 0.01, for Grn⁺ versus CT and Grn^–, n = 3 per group.

Fig. 9. Aortic relaxation function in the various experimental groups: Sprague–Dawley (SD) on control diet (CT), SD on glucoraphanin-containing broccoli sprout diet (Grn⁺), as well as SHRsp on CT, Grn⁺ diet, and glucoraphanin-depleted sprout diet (Grn^–); n = 9 per group. Aortic rings were precontracted with phenylephrine, and tension was measured in the presence of various concentrations of sodium nitroprusside, a nitric oxide donor. Sodium nitroprusside caused efficient relaxation in both SD diet groups with a significantly less response in all SHRsp diet groups. *, P < 0.05, for SD diet groups versus SHRsp diet groups.

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