An alternative agriculture system is defined by a distinct expression profile of select gene transcripts and proteins

Kumar et al. 10.1073/pnas.0403496101.

Supporting Information

Files in this Data Supplement:

Supporting Table 2
Supporting Figure 6
Supporting Table 3




Fig. 6. Visual status of fresh market tomato plants cultivated in the field on either black polyethylene (BP) (Upper) or hairy vetch (HV) (Lower) mulch at 82, 96 and 108 days after planting.





Table 2. Sequence of primers used for PCR-derived probes

Gene

Forward primer

Reverse primer

GenBank

Product size, bp

NiR

5’-GGATACAGCTTTGTTGGT-3’

5’-CTTGTCCACATGTATTTGG-3’

BG791272

379

G6PD

5’-AACCCTTTATTCTTCTC-3’

5’-ATGTTCCTGAGTTCGTCA-3’

AW035603

446

Osmotin

5’-GAAGTGACTCAATTTTA-3’

5’-CCTGGCCGAGTACGCCTT-3’

AW218786

451

psbA

5’-ATGACTGCAATTTTAGAG-3’

5’-GATCAAGAAAACAGCAGTCG-3’

AP000423

480

PAL

5’-CAACACTCTGCTTCAAGGCT-3’

5’-TGGAGATGTTCGGAGAGCAT-3’

M83314

580

PR1A

5’-GGTTGTTCAACATCTCATTG-3’

5’-GTTGCAACGAGCCCGACCAC-3’

AJ011520

407

b-tubulin

5’-AGTATCAGATCTGGG-3’

5’-AAATCTGAGGCAGCAAG-3’

BF096939

505

ACS6

5’-ATGGGGTTAATTTCAAAGAT-3’

5’-AACTCTCACAAACAACAGGA-3’

AF179249

502

CKR

5’-TGCTGATGTTATTCATGCCA-3’

5’-CAAGATTATACAGCTTTGAC-3’

AI774919

420

GA20-oxidase

5’-ATGGCTATTGATTGTATGA-3’

5’-TTTGAAGTGACTCTTCTCTA-3’

AF049898

636





Table 3. Relative band intensities for each transcript shown in Fig. 3 were determined by normalizing against b-tubulin band intensity

Gene

Transcript

BP

(108/96)

HV

(108/96)

rbcS

0.64

0.85

rbcL

0.57

1.07

GS1

0.26

1.04

psbA

1.01

1.01

NiR

0.53

1.20

G6PD

1.00

1.50

chiB

0.25

0.97

osmotin

0.91

1.18

chiA

0.97

1.05

hsp70

0.15

0.86

BiP

0.48

1.05

SAG12

1.44

1.20

CHL1

1.00

1.08

The ratio of normalized signals obtained for day 108 to that on day 96 for each transcript is given.

This Article

  1. PNAS July 20, 2004 vol. 101 no. 29 10535-10540
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