A family of Acrp30/adiponectin structural and functional paralogs

Wong et al. 10.1073/pnas.0403760101.

Supporting Information

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Supporting Table 2
Supporting Figure 6
Supporting Table 3
Supporting Figure 7
Supporting Figure 8
Supporting Figure 9

Supporting Figure 6

Fig. 6. CTRP2 is highly conserved throughout evolution. The full-length or partial amino acid sequences of CTRP2 present in human, mouse, rat, bovine, pig, chicken, fugu, and zebrafish were extracted from the GenBank databases and aligned by using the CLUSTALW program. Identical amino acids are shaded black and similar amino acids are shaded gray. Numbering of amino acids is located on the right. The collagenous and C-terminal globular domains are indicated by the arrows. l indicates the conserved cysteine residues potentially involved in forming higher-order oligomers through disulfide bonding.

Supporting Figure 7

Fig. 7. Comparison of the C-terminal globular C1q-like domain of adiponectin and mCTRP1, mCTRP2, mCTRP3, mCTRP5, mCTRP6, and mCTRP7. The amino acid sequences of the C-terminal globular domain of adiponectin, mCTRP1, mCTRP2, mCTRP3, mCTRP5, mCTRP6, and mCTRP7 were extracted from the GenBank databases and aligned by using the CLUSTALW program. Identical amino acids are shaded black and similar amino acids are shaded gray. Arrows indicate four residues (Gly-159, Tyr-161, Phe-237, and Leu-242; adiponectin numbering) conserved throughout both C1q (adiponectin and complement C1q) and TNF (TNF-a, TNF-b , and CD40L) families of proteins.

Supporting Figure 8

Fig. 8. Structure of the mCTRP genes. The chromosome location and the exon/intron organization of adiponectin gene were compared with that of mCTRP genes. CTRP genes sequences were downloaded from GenBank databases and analyzed individually to confirm the exon/intron boundaries and the sizes of each exon and intron. Boxes (■) indicate exons. The chromosomal location and the size of each gene are indicated on the left.

Supporting Figure 9

Fig. 9. Expression of mCTRP1, mCTRP3, mCTRP7, and adiponectin in mouse adipose tissue. RT-PCR analysis of mCTRP1, mCTRP3, mCTRP7, and adiponectin transcripts were carried out on RNA isolated from three separate wild-type (WT) or obese (ob/ob) mouse adipose tissue. Thirty-five cycles of PCR were performed. RT-PCR analysis of HPRT transcript was carried out to show the presence of RNA in each sample.