Fusogenic supramolecular vesicle systems induced by metal ion binding to amphiphilic ligands

Richard et al. 10.1073/pnas.0406625101.

Supporting Information

Files in this Data Supplement:

Supporting Table 1
Supporting Figure 6
Supporting Movie 1
Supporting Figure 7
Supporting Figure 8

Supporting Figure 6

Fig. 6. Images of large unilamellar vesicles containing 3 mol % lipoligand B10 obtained from cryofracture electron microscopy before incubation in the presence of NiCl₂.

Supporting Figure 7

Fig. 7. UV spectra of a suspension of vesicles containing 3 mol % lipoligand B14 before (□) and after addition of various divalent cations, Ni²⁺ (▲) , Co²⁺ (○), or Ca²⁺ (♦), and after an incubation time of 30 min.

Supporting Figure 8

Fig. 8. Optical phase-contrast microscopy images of vesicles containing x mol % B14 after 1 h of incubation in the presence of 0.1 mM NiCl₂. (a) x = 1. (b) x = 2. (c) x = 3. (Scale bar, 10 mm.)

Supporting Movie 1

Movie 1. Movie of the fusion between two giant vesicles containing 3 mol % lipoligand B10 obtained by addition of NiCl₂ (0.1 mM) and followed by optical phase-contrast microscopy in real time.

Table 1. Temperatures and heats of transition (in parentheses) observed for multilamellar vesicle suspensions composed of various mixtures in pure water as measured by differential scanning calorimetry

System	Bn
System	B4	B10	B14
Pure	50°C (130 kJ/mol)	45°C (190 kJ/mol)	44°C (220 kJ/mol)
In water	48°C (130 kJ/mol)	37°C (177 kJ/mol)	29-38°C (154 kJ/mol)
Bn/EPC (5:95)	34.5°C (132 kJ/mol)	No peak	No peak