Impaired up-regulation of CD25 on CD4 + T cells in IFN-γ knockout mice is associated with progression of myocarditis to heart failure

Afansyeva et al. 10.1073/pnas.0408241102.

Supporting Information

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Supporting Figure 5
Supporting Table 1





Supporting Figure 5

Fig. 5. IFN-g deficiency leads to more severe myocarditis. (a and b) Gross and histologic scores respectively in WT (filled symbols) and IFN-g knockout (KO; open symbols) mice on days 21–24, 31–33, 42–44, and 58–62 after immunization. P values were derived by using the Mann–Whitney U test. (c) Percentage of heart-infiltrating leukocytes (CD45+ cells) of total heart cells in unimmunized (day 0) and immunized (days 15, 21–24, 30–34, and 58–62 after immunization) WT (filled symbols) and IFN-g KO (open symbols) mice. P values were derived by using Student’s t test. Each symbol represents an individual mouse.





Table 1. Expansion of CD3+CD44high T cells in peripheral blood in immunized IFN-g KO mice

 

Day 3

Day 9

Day 16

Cells

WT

KO

WT

KO

WT

KO

CD3+

61.4 ± 3.2

59.1 ± 2.2

64.5 ± 4.1

69.1 ± 5.2

37.2 ± 8.8

54.0 ± 5.4*

CD3+CD44high

8.3 ± 0.6

10.2 ± 0.6*

13.4 ± 0.5

20.1 ± 2.1 *

12.2 ± 1.3

29.3 ± 5.1*

Ann V+7AAD-

not done

not done

3.3 ± 0.3

2.0 ± 0.2 *

5.7 ± 0.6

4.1 ± 1.4

KO, IFN-g knockout; Ann V, annexin V; 7AAD, 7-aminoactinomycin D. Data are presented as percentage positive cells of total lymphocyte population for CD3+ or CD3+CD44high cells and of CD3+ population for annexinV+7AAD– cells. Data are shown as mean ± SD and represent three mice per group. *, P < 0.05, Student's t test, vs. WT.

This Article

  1. Published online before print December 20, 2004, doi: 10.1073/pnas.0408241102 PNAS January 4, 2005 vol. 102 no. 1 180-185
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