Changes in DNA bending induced by restricting nucleotide ring pucker studied by weak alignment NMR spectroscopy
Wu et al. 10.1073/pnas.0408498102.
Supporting Information
Files in this Data Supplement:
Supporting Table 3Table 3. Experimental residual dipolar couplings in 20 ± 2 mg/ml Pf1
|
T8* |
T7* |
T7*T8* |
Native |
|
|
C1 H5-C5 |
18.27 ± 1.00 |
18.68 ± 1.20 |
23.19 ± 2.05 |
17.04 ± 1.00 |
|
C1 H6-C6 |
14.23 ± 1.13 |
14.45 ± 1.25 |
14.33 ± 1.50 |
11.14 ± 1.47 |
|
C1 H1′-C1′ |
1.08 ± 1.00 |
2.30 ± 1.00 |
2.84 ± 1.00 |
1.29 ± 1.00 |
|
C1 H3′-C3′ |
-5.45 ± 1.00 |
-3.94 ± 1.00 |
-5.12 ± 1.00 |
-5.84 ± 1.00 |
|
C1 H4′-C4′ |
5.67 ± 1.00 |
8.08 ± 1.00 |
13.95 ± 1.00 |
4.91 ± 1.00 |
|
G2 H8-C8 |
12.70 ± 1.00 |
12.33 ± 1.03 |
7.75 ± 1.12 |
9.81 ± 1.25 |
|
G2 H1′-C1′ |
18.18 ± 1.00 |
18.13 ± 1.00 |
20.55 ± 1.04 |
20.81 ± 1.00 |
|
G2 H3′-C3′ |
7.59 ± 1.03 |
6.32 ± 1.00 |
3.62 ± 1.49 |
|
|
G2 H4′-C4′ |
7.35 ± 1.00 |
13.02 ± 1.00 |
1.30 ± 1.00 |
|
|
C3 H5-C5 |
12.20 ± 1.78 |
12.28 ± 1.48 |
11.78 ± 2.90 |
10.01 ± 1.62 |
|
C3 H6-C6 |
19.69 ± 1.85 |
18.17 ± 2.26 |
17.91 ± 2.34 |
14.95 ± 2.29 |
|
C3 H1′-C1′ |
4.91 ± 1.00 |
4.10 ± 1.00 |
-0.12 ± 1.00 |
4.90 ± 1.00 |
|
C3 H3′-C3′ |
1.60 ± 1.06 |
-8.78 ± 1.62 |
||
|
C3 H4′-C4′ |
-4.82 ± 1.00 |
-6.20 ± 1.38 |
||
|
G4 H8-C8 |
20.34 ± 1.24 |
18.01 ± 1.34 |
||
|
G4 H1′-C1′ |
19.47 ± 1.00 |
20.54 ± 1.00 |
||
|
G4 H3′-C3′ |
14.45 ± 1.23 |
|||
|
G4 H4′-C4′ |
-3.47 ± 1.00 |
|||
|
A5 H2-C2 |
20.67 ± 1.43 |
|||
|
A5 H8-C8 |
18.63 ± 1.72 |
|||
|
A5 H1′-C1′ |
20.75 ± 1.00 |
|||
|
A5 H3′-C3′ |
19.99 ± 1.00 |
|||
|
A5 H4′-C4′ |
-6.84 ± 1.03 |
|||
|
A6 H2-C2 |
18.74 ± 1.83 |
|||
|
A6 H8-C8 |
17.88 ± 2.31 |
|||
|
A6 H1′-C1′ |
20.26 ± 1.00 |
|||
|
A6 H3′-C3′ |
19.86 ± 1.00 |
|||
|
A6 H4′-C4′ |
4.14 ± 1.17 |
|||
|
T7 H6-C6 |
17.61 ± 3.31 |
|||
|
T7 H1′-C1′ |
17.48 ± 1.00 |
|||
|
T7 H4′-C4′ |
8.32 ± 1.23 |
|||
|
T8 H6-C6 |
19.30 ± 2.99 |
|||
|
T8 H1′-C1′ |
18.60 ± 1.00 |
|||
|
T8 H3′-C3′ |
21.36 ± 1.09 |
|||
|
T8 H4′-C4′ |
11.26 ± 1.05 |
|||
|
C9 H5-C5 |
21.07 ± 1.65 |
20.30 ± 1.44 |
||
|
C9 H6-C6 |
21.66 ± 2.44 |
20.35 ± 2.61 |
||
|
C9 H1′-C1′ |
13.73 ± 1.00 |
13.24 ± 1.00 |
||
|
C9 H3′-C3′ |
17.59 ± 1.00 |
21.43 ± 1.00 |
||
|
C9 H4′-C4′ |
||||
|
G10 H8-C8 |
21.75 ± 1.00 |
16.56 ± 1.15 |
18.47 ± 1.10 |
18.23 ± 1.18 |
|
G10 H1′-C1′ |
20.24 ± 1.00 |
19.35 ± 1.00 |
25.05 ± 1.30 |
15.12 ± 1.00 |
|
G10 H3′-C3′ |
16.08 ± 1.00 |
15.04 ± 1.00 |
10.71 ± 1.36 |
|
|
G10 H4′-C4′ |
16.37 ± 1.13 |
24.64 ± 1.04 |
||
|
C11 H5-C5 |
23.47 ± 2.06 |
20.42 ± 1.62 |
23.65 ± 2.63 |
22.36 ± 1.28 |
|
C11 H6-C6 |
14.33 ± 1.50 |
11.19 ± 2.17 |
10.75 ± 2.75 |
12.56 ± 1.98 |
|
C11 H1′-C1′ |
13.51 ± 1.00 |
11.07 ± 1.00 |
12.16 ± 1.00 |
13.88 ± 1.00 |
|
C11 H3′-C3′ |
7.11 ± 1.00 |
3.57 ± 1.00 |
-0.48 ± 1.00 |
9.49 ± 1.00 |
|
C11 H4′-C4′ |
14.51 ± 1.00 |
12.62 ± 1.21 |
||
|
G12 H8-C8 |
8.17 ± 1.00 |
6.84 ± 1.00 |
3.70 ± 1.00 |
4.84 ± 1.00 |
|
G12 H1′-C1′ |
14.44 ± 1.00 |
12.91 ± 1.00 |
13.03 ± 1.00 |
15.57 ± 1.00 |
|
G12 H3′-C3′ |
7.83 ± 1.00 |
5.97 ± 1.00 |
3.53 ± 1.00 |
7.56 ± 1.00 |
|
G12 H4′-C4′ |
12.31 ± 1.00 |
9.49 ± 1.00 |
8.27 ± 1.00 |
14.41 ± 1.00 |
Errors in the reported dipolar couplings correspond to uncertainties estimated on the basis of the observed line width and south/north for the corresponding resonances in the isotropic and aligned media, according to Kontaxis et al. (1). Errors smaller than 1 Hz are rounded up to 1 Hz.
1. Kontaxis, G., Clore, G. M. & Bax, A. (2000) J. Magn. Reson. 143, 184-196
This Article
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PNAS January 4, 2005 vol. 102 no. 1 24-28
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