Directing cell migration with asymmetric micropatterns

Jiang et al. 10.1073/pnas.0408954102.

Supporting Information

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Supporting Figure 5
Supporting Figure 6
Supporting Figure 7
Supporting Figure 8
Supporting Figure 9
Supporting Figure 10
Supporting Figure 11
Supporting Figure 12
Supporting Figure 13
Supporting Figure 14
Supporting Figure 15

Supporting Figure 5

Fig. 5. The motility of 3T3 fibroblasts initially confined to narrow drops.

Supporting Figure 6

Fig. 6. The motility of 3T3 fibroblasts initially confined to wide drops.

Supporting Figure 7

Fig. 7. 3T3 fibroblasts confined to rectangles that have the same aspect ratio and area as the narrow drop move into regions a and b with equal likelihood but rarely move into region c.

Supporting Figure 8

Fig. 8. 3T3 fibroblasts confined to squares move randomly.

Supporting Figure 9

Fig. 9. 3T3 fibroblasts confined to circles move randomly.

Supporting Figure 10

Fig. 10. 3T3 fibroblasts confined to elongated triangles move directionally. The circle for grouping these cells is rotated 13° counterclockwise for this case, because the orientation of the cells is rotated to the same extent.

Supporting Figure 11

Fig. 11. A series of patterns that confine cells to approximately the same projected geometry (visualized by the actin cytoskeleton) but distribute the focal adhesions (FAs; visualized by immunostaining for vinculin) differently. The bottom row shows that new focal adhesions formed 1 h after release in areas that were inert to attachment of cells prior to release (arrowheads).

Supporting Figure 12

Fig. 12. 3T3 fibroblasts confined to V-shaped patterns move directionally.

Supporting Figure 13

Fig. 13. 3T3 fibroblasts confined to L-shaped patterns move directionally.

Supporting Figure 14

Fig. 14. 3T3 fibroblasts confined to A-shaped patterns move directionally.

Supporting Figure 15

Fig. 15. 3T3 fibroblasts confined to teardrop-shaped patterns were initially treated with 5 m M nocodazole (noco) and then electrochemically released, immediately followed by rinsing away the nocodazole.