Gelsolin mediates calcium-dependent disassembly of Listeria actin tails

Larson et al. 10.1073/pnas.0409062102.

Supporting Information

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Supporting Movie 1
Supporting Movie 2




Supporting Movie 1

Movie 1. Time-lapse fluorescence images of Listeria-infected MDCK cells transfected with EGFP-actin showing the formation of tails by motile intracellular bacteria. The bacteria cannot be seen in the fluorescence image and are directly in front of each tail. Video was taken 3.5 h after initiation of infection for this control condition (see Fig. 1A). Images were captured at a frequency of one frame per 10 s and displayed at a rate of 6 frames per s.





Supporting Movie 2

Movie 2. Time-lapse fluorescence images of the same Listeria-infected MDCK cells shown in Movie 1 after 20 min of exposure to BAPTA-AM and EGTA (see Fig. 1B). Cells were treated with 40 m M BAPTA-AM and 1 mM EGTA (see Results). Images were captured at a frequency of one frame per 10 s and displayed at a rate of 6 frames per s. Note the marked lengthening of the actin filament tails.

This Article

  1. Published online before print January 25, 2005, doi: 10.1073/pnas.0409062102 PNAS February 8, 2005 vol. 102 no. 6 1921-1926
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