In vitro and in vivo two-photon luminescence imaging of single gold nanorods

Wang et al. 10.1073/pnas.0504892102.

Supporting Movies

Files in this Data Supplement:

Movie 1. Two-photon luminescence (TPL) intensity changes with rotating incident polarization (pseudocolor). The incident beam is linearly polarized. For single nanorods, the TPL intensity displays a cos⁴ dependence on the excitation polarization. For a ring-like cluster of nanorods, different regions of the ring are illuminated as the excitation polarization is rotated. The intensity pattern suggests that the nanorods are oriented tangential to the ring perimeter. The image size is 25 × 25 mm.

Movie 2. TPL intensity changes with the rotating polarizer placed before the photomultiplier tube (PMT) detector (pseudocolor). The intensities from individual spots are nearly the same with some minor random changes, indicating that the TPL emissions are depolarized.

Movie 3. TPL imaging of gold nanorods (pseudored dots) flowing in mouse ear blood vessels. The movie contains 300 frames with an acquisition rate of 1.12 sec per frame; image size is 175 × 175 mm. The blue color represents the simultaneously acquired transmission image.

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