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Biddle et al. 10.1073/pnas.0600035103.

Supporting Information

Files in this Data Supplement:

Supporting Figure 4
Supporting Figure 5
Supporting Table 3
Supporting Table 4
Supporting Text




Supporting Figure 4

Fig. 4. Concentrations of sulfate (open squares), methane (diamonds), and counted cells after staining with acridine orange (AODC; crosses) (1). SMTZs are marked by gray bars.

1. D’Hondt, S. L., Jørgensen, B. B., Miller, D. J. & Shipboard Scientific Party (2003) Proceedings of the Ocean Drilling Program: Initial Reports (Ocean Drilling Program, Texas A & M University, College Station, TX), Vol. 201.





Supporting Figure 5

Fig. 5. 16S rRNA archaeal phylogenetic tree based on maximum likelihood distances of » 900 nucleotide positions (16S rRNA positions 23–914). Compared with Fig. 1, this tree includes a greater variety of reference sequences from different habitats and shows groups of closely related Peru Margin subsurface sequences in finer resolution. As previously, bootstrap numbers are based on 200 resamplings. The sequences were obtained from four sediment samples from each of the four SMTZs encountered during ODP Leg 201: 1227D-37.8 (ODP Hole-depth in hole in meters below seafloor), 1229D-29.4, 1229D-86.8, and 1230A-11.0. Closely related sequence clusters are represented by single sequences in the tree, annotated with the number of near-identical 16S rRNA clones that they represent, and their GenBank accession numbers. Sequence names consist of sample name and GenBank accession numbers. Sequences are color coded by habitat to illustrate the diverse environmental range of these uncultured archaeal lineages.





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