Micromechanical control of cell–cell interactions

Hui and Bhatia. 10.1073/pnas.0608660104.

Supporting Information

Files in this Data Supplement:

SI Movie 1
SI Appendix
SI Figure 6




Fig. 6. Micromechanical substrates can be generalized to other cell types and biological techniques. (A) Bipotential mouse embryonic liver progenitor cells cultured for 1 day on a micromechanical substrate. The BMEL cell line, 9A1, was provided by Mary Weiss (Institut Pasteur, Paris) and cultured as described (1, 2). In brief, cells were cultured on collagen in RPMI medium 1640 with glutamax (Invitrogen, Carlsbad, CA), containing 30 ng/ml human IGF-II (Peprotech, Rocky Hill, NJ), 50 ng/ml human EGF (Peprotech), and 10 mg/ml recombinant human insulin (Invitrogen). (B) Primary rat liver sinusoidal endothelial cells (LSEC) cultured for 1 day on a collagen-coated micromechanical substrate. Briefly, LSEC were isolated from the nonparenchymal fraction of the liver through a 25%/50% Percoll gradient (3) and cultured in the presence of VEGF (R&D Systems, Minneapolis, MN). (C) OP9 bone marrow stromal cells (ATCC, Manassas, VA) cultured for 1 day on a collagen-coated micromechanical substrate, using alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine and 1.5 g/liter sodium bicarbonate, 80%; FBS, 20% (all from Invitrogen). (D) Cells can be transfected with siRNA while adhered to micromechanical substrates, allowing selective delivery using the separated mode. Fluorescent image of Swiss 3T3 fibroblasts transfected with siRNA sequence (against Lamin A) with FITC fluorophore conjugated to 5' end of sense strand (Dharmacon, Lafayette, CO). Transfection was performed by using Lipofectamine 2000 (Invitrogen) on cells adhered to the substrate.

1. Strick-Marchand H, Morosan S, Charneau P, Kremsdorf D, Weiss MC (2004) Proc Natl Acad Sci USA 101:8360-8365.

2. Strick-Marchand H, Weiss MC (2002) Hepatology 36:794-804.

3. Zhang B, Borderie D, Sogni P, Soubrane O, Houssin D, Calmus Y (1997) J Hepatol 26:1348-1355.





SI Movie 1

Movie 1. Actuation of micromechanical substrate. Parts begin fully separated and are pushed manually to the gap mode, then to the contact mode, and finally separated again. Note that the parts are firmly attached in the gap and contact modes, remaining fixed together even when the parts are jostled.

This Article

  1. Published online before print March 27, 2007, doi: 10.1073/pnas.0608660104 PNAS April 3, 2007 vol. 104 no. 14 5722-5726
  1. AbstractFree
  2. Figures Only
  3. Full Text
  4. Full Text (PDF)
  5. » Supporting Information
About Our New Site Design >>
Link: Advanced Search

This Week's Issue

  1. July 22, 2008, 105 (29)
  1. Current Issue
  1. Alert me to new issues of PNAS

Most