Magnetic microposts as an approach to apply forces to living cells

Sniadecki et al. 10.1073/pnas.0611613104.

Supporting Information

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SI Movie 1
SI Figure 5
SI Movie 2




SI Figure 5

Fig. 5. FA size increased with external force from micromanipulation with a pulled glass microneedle. (A) Phase-contrast micrograph of a representative cell on nonmagnetic posts. The microneedle is in the upper right corner of the image. (B) Micrograph of a cell after applying an external force by pulling a post with the microneedle. After 10 min of force application with the microneedle, cells were fixed and stained for vinculin. (C) Plot of average FA area per post for all posts underneath the cells and at the posts that were mechanically pulled (‡, P = 0.09; n = 3 cells).





SI Movie 1

Movie 1. Phase-contrast microscopy video of a magnetic micropost actuated under an applied field that was cycled between -0.31 T and 0.31 T.





SI Movie 2

Movie 2. Phase-contrast microscopy video of traction force dynamics of cell A that was force-stimulated at t = 0 min. Red arrows denote direction and magnitude of traction forces for cell A, and blue arrows correspond to apparent forces at background posts. Posts of interest for cell A (Ai), which were previously shown in Fig. 4A, are denoted in the first video frame.

This Article

  1. Published online before print September 5, 2007, doi: 10.1073/pnas.0611613104 PNAS September 11, 2007 vol. 104 no. 37 14553-14558
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