Epigenetic silencing of a Ca²⁺-regulated Ras GTPase-activating protein RASAL defines a new mechanism of Ras activation in human cancers

Jin et al. 10.1073/pnas.0700153104.

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Fig. 8. PITX1 expression is not down-regulated in those cell lines showing a reduced level of RASAL. PITX1 expression in a variety of cell lines was examined by semiquantitative RT-PCR, with GAPDH as a control. The corresponding levels of RASAL expression are also indicated for each cell line (see Fig. 2A).

Fig. 9. Ectopic RASAL expression inhibits tumor cell growth through Ras inactivation. (A) The effect of ectopic expression of RASAL and its mutants on tumor cell growth was investigated by monolayer colony formation assay. Quantitative analysis of colony numbers is shown in Bottom as values of mean ± standard deviation. (B) The effect of RASAL expression on tumor cell growth in the presence of wild type (wt) or mutant (mu) RAS(Q61L) was investigated by soft agar assay. Results are summarized in Fig. 7C.