Nucleomorph genome of Hemiselmis andersenii reveals complete intron loss and compaction as a driver of protein structure and function

Lane et al. 10.1073/pnas.0707419104.

Supporting Information

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SI Figure 4
SI Table 1
SI Figure 5

SI Figure 4

Fig. 4. Comparison of gene content between the Hemiselmis andersenii and Guillardia theta nucleomorph genomes. Genes are listed according to functional category, with presence/absence indicated by color. In cases where a gene in H. andersenii is homologous to an unnamed gene in G. theta, the name of the G. theta ORF is given in parentheses. RNA metabolism genes with predicted functions related to mRNA splicing and the spliceosome are underlined.

SI Figure 5

Fig. 5. Select partial alignments of diverse eukaryotic protein sequences showing amino- and/or carboxy-terminal (a and b) and internal (c-e) shortening of the Hemiselmis andersenii and/or Guillardia theta nucleomorph-encoded proteins compared to their homologs in other eukaryotes. Shortening is also apparent in the proteins of the microsporidian Encephalitozoon cuniculi. Deletions are highlighted with arrowheads. Invariant amino acid residues are shaded black, residues present in >60% of the sequences are dark blue and conservative substitutions are light blue. In each case, the amino acid coordinates correspond to the Drosophila melanogaster protein.