Cost-effective production of a vaginal protein microbicide to prevent HIV transmission

Ramessar et al. 10.1073/pnas.0708841104.

Supporting Figures

Files in this Data Supplement:

SI Figure 3
SI Figure 4
SI Figure 5
SI Figure 6

SI Figure 3

Fig. 3. Dot blot detection of the 2G12 heavy (A) and light (B) chain in endosperm tissue of T₁ transgenic seeds. Five individual seeds (columns 1-5) per event (labeled per row) were tested. Color intensity is indicative of level of expression. Positive (+C) and negative (-C) controls are indicated.

SI Figure 4

Fig. 4. Dot blot detection of the 2G12 heavy (A) and light (B) chain in endosperm tissue of T₂ transgenic seeds. Six individual seeds (columns 1-6) per progeny (labeled per row) were tested. Color intensity is indicative of level of expression. Positive controls are indicated.

SI Figure 5

Fig. 5. Biacore measurements of 2G12 levels in 10 individual T₂ seeds from events 1G (low expression) and 3C (highest expression), indicating variation in expression levels among seeds from the same lineage (protein A represents amount of heavy chain detected; protein L represents amount of light chain detected). All seeds tested were homozygous by analysis of T₃ populations derived from each seed.

SI Figure 6

Fig. 6. Analysis of the quality of maize-derived antibody preparation by surface plasmon resonance spectroscopy binding assays. Binding signals were simultaneously recorded on protein-A, protein-L and gp120 (antigen) surfaces. The blank subtracted binding signals were plotted pair wise and subjected to linear regression analysis to determine the binding signal ratios. (A) protein-L/protein-A ratio. (B) gp120/protein-A ratio. (C) gp120/protein-L ratio.