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Sangster et al. 10.1073/pnas.0712210105.

Supporting Information

Files in this Data Supplement:

SI Figure 3
SI Table 3
SI Figure 4
SI Figure 5
SI Table 4
SI Table 5
SI Table 6
SI Figure 6
SI Table 7




SI Figure 3

Fig. 3. Construction of HSP90-reduced RILs. The same breeding method was used to create a control RIL set.





SI Figure 4

Fig. 4. Graphical genotype maps of RIL sets. Each RIL is a vertical column. (Upper) HSP90-RNAi RILs. (Lower) Control RILs. Chromosomes (Chr) 1-5 top to bottom. Red, scored as Col-0; blue, scored as Ler; yellow, not scored. Arrows point to locations of construct inserts and concomitant segregation distortion.





SI Figure 5

Fig. 5. QTL affecting hypocotyl and root length in HSP90-reduced and control RILs. Red, HSP90-reduced set; green, control set. The upper portion for each trait represents QTL significance in LOD; the horizontal line of the appropriate color denotes a genome-wide a = 0.05 significance threshold. Both plots are interval maps. The lower portion for each trait represents estimated additive effect from the Col-0 allele to the midpoint.





SI Figure 6

Fig. 6 Significant QTL detected. Red, HSP90-RNAi set; green, control set. Stars, significantly HSP90-responsive QTL; black bar, most significant location for QTL; box, 2-LOD interval. QTL significance is in shades of gray. Additive effect by direction of arrow, up signifies Col-0 allele increases the trait mean. Trait variance explained by QTL is indicated by size of arrow. Trait identifier key:

1:

Days from germination to flowering

2:

Rosette leaf number

3:

Cauline leaf number

4:

Total leaf number

5:

Longest leaf length

6:

Longest leaf width

7:

Longest leaf depth

8:

Longest leaf petiole length

9:

Longest leaf petiole length/longest leaf length

10:

Longest leaf surface area

11:

Longest leaf volume

12:

Internode mean

13:

Plant height

14:

Days actively flowering

15:

Growth per day

16:

Primary stem fertile siliques

17:

Primary stem infertile siliques

18:

Primary stem total siliques

19:

Secondary stems from rosette

20:

Secondary stems off primary stem

21:

Dry mass of aerial tissue

22:

Total seed weight

23:

Total seed weight/dry mass





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