Multiple sclerosis: Brain-infiltrating CD8 + T cells persist as clonal expansions in the cerebrospinal fluid and blood

Table 2. TCR-BV sequences identified in brain tissue, CSF cells, and sorted peripheral blood cells (PBL) from Patient A by CDR3 spectratyping

TCR-BV CDR3 sequence	Brain 1996	CSF 2001	CD4⁺ PBL 2001	CD8⁺ PBL 2001	CD38⁺ PBL 2001
BV9 - C A S S - P W E E G - S G N - BJ1.3	+	+	-	-	-
BV9 - C A S S - Q I R G V G - E Q Y - BJ2.7	+	(+)^#	-	-	+
BV16 - C A S - G G T S G K - D T Q - BJ2.3	+	(+)	-	-	-
BV17 - C A S S - W A I S D R - Q P Q - BJ1.5	+	(+)	-	-	-

The N-D-N regions are shown in italics, +, completely readable sequence; (+), clonal identity clearly discernible on an oligoclonal background; (+)^#, completely readable sequence with clone-specific primer; -, clone not identifiable by CDR3 spectratyping and/or direct sequencing. Note that the TCR sequences observed at different sites and time points were identical both at the nucleotide and amino acid levels