Multiple sclerosis: Brain-infiltrating CD8 + T cells persist as clonal expansions in the cerebrospinal fluid and blood

Table 3. TCR-BV sequences identified in brain tissue, CSF cells, and sorted peripheral blood cells (PBL) from patient B by CDR3 spectratyping

TCR-BV CDR3 sequence	Brain 05/2001	CSF 11/2001	CD4⁺ PBL 12/2001	CD8⁺ PBL 12/2001	CD38⁺ PBL 12/2001
BV16 - C A S S - R L T D S T - T D T - BJ2.3	(+)	-	-	+	-
BV19 - C A S S - H P G T G S N - E Q Y - BJ2.7	+	+	-	(+)	-
BV20 - C A W S - A P D - N T E - BJ1.1	(+)	-	-	(+)	-
BV23 - C A S S - P G R A G - S P L - BJ1.6	-	+	+	-	-
BV24 - C A T S - R L Q G G - T G E - BJ2.2	+	+	-	-	+

The N-D-N regions are shown in italics, +, completely readable sequence; (+), clonal identity clearly discernible on an oligoclonal background; -, clone not identifiable by CDR3 spectratyping and/or direct sequencing. Note that the TCR sequences observed at different sites and time points were identical both at the nucleotide and amino acid levels.