IgG glycan hydrolysis by a bacterial enzyme as a therapy against autoimmune conditions
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Fig. 4.
Rabbit antibody response to EndoS. Serum samples were withdrawn from the rabbit at indicated time points after the first (0 days), second (35 days), and third (135 days) injections of EndoS. Serum samples after the first (0 days), second (35 days), and third (135 days) injections were used as primary antisera in an ELISA experiment with immobilized EndoS. Increase in concentration (ng/ml) of anti-EndoS IgG compared with concentration before first injection is presented. One representative experiment is shown. (Inset) The serum samples after the first injection were used as primary antisera in a Western blot on separate membrane strips with SDS/PAGE-separated purified EndoS.
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PNAS March 18, 2008 vol. 105 no. 11 4265-4270
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