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Published online on February 19, 2008, 10.1073/pnas.0800053105
PNAS | February 26, 2008 | vol. 105 | no. 8 | 2975-2980
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BIOLOGICAL SCIENCES / GENETICS
HSP90/70 chaperones are required for rapid nucleosome removal upon induction of the GAL genes of yeast

Monique Floer, Gene O. Bryant, and Mark Ptashne*

Molecular Biology Program, Sloan–Kettering Institute, 1275 York Avenue, New York, NY 10021

Contributed by Mark Ptashne, January 4, 2008 (received for review December 19, 2007)

Induction of transcription of the GAL genes of yeast by galactose is a multistep process: Galactose frees the activator Gal4 of its inhibitor, Gal80, allowing Gal4 to recruit proteins required to transcribe the GAL genes. Here, we show that deletion of components of either the HSP90 or the HSP70 chaperone machinery delays this induction. This delay remains when the galactose-signaling pathway is bypassed, and it cannot be explained by a chaperone requirement for DNA binding by Gal4. Removal of promoter-bound nucleosomes is delayed in a chaperone mutant, and our findings suggest an involvement of HSP90 and HSP70 in this early step in gene induction.

transcription | Gal4 | activation | recruitment | promoter

Freely available online through the PNAS open access option.

Author contributions: M.F., G.O.B., and M.P. designed research; M.F. and G.O.B. performed research; M.F. and G.O.B. analyzed data; and M.F., G.O.B., and M.P. wrote the paper.

The authors declare no conflict of interest.

*To whom correspondence should be addressed. E-mail: m-ptashne{at}ski.mskcc.org

© 2008 by The National Academy of Sciences of the USA


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