Previous Article |
Table of Contents
| Next Article 
DNA Sequencing with Chain-Terminating Inhibitors
A new method for determining nucleotide sequences in DNA is described. It is similar to the ``plus and minus'' method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage
Copyright © 1977 by the National Academy of Sciences
X174 and is more rapid and more accurate than either the plus or the minus method.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg What's this?
This article has been cited by other articles in HighWire Press-hosted journals:
|
|
 |
|
  H. C. Zaun, A. Shrier, and J. Orlowski Calcineurin B Homologous Protein 3 Promotes the Biosynthetic Maturation, Cell Surface Stability, and Optimal Transport of the Na+/H+ Exchanger NHE1 Isoform J. Biol. Chem., May 2, 2008; 283(18): 12456 - 12467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. M. Abd-Alla, F. Cousserans, A. G. Parker, J. A. Jehle, N. J. Parker, J. M. Vlak, A. S. Robinson, and M. Bergoin Genome Analysis of a Glossina pallidipes Salivary Gland Hypertrophy Virus Reveals a Novel, Large, Double-Stranded Circular DNA Virus J. Virol., May 1, 2008; 82(9): 4595 - 4611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Letek, E. Ordonez, J. Vaquera, W. Margolin, K. Flardh, L. M. Mateos, and J. A. Gil DivIVA Is Required for Polar Growth in the MreB-Lacking Rod-Shaped Actinomycete Corynebacterium glutamicum J. Bacteriol., May 1, 2008; 190(9): 3283 - 3292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Jimenez, R. Canals, A. Lacasta, A. N. Kondakova, B. Lindner, Y. A. Knirel, S. Merino, M. Regue, and J. M. Tomas Molecular Analysis of Three Aeromonas hydrophila AH-3 (Serotype O34) Lipopolysaccharide Core Biosynthesis Gene Clusters J. Bacteriol., May 1, 2008; 190(9): 3176 - 3184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zhou, M. Lei, Y. Rao, Q. Nie, H. Zeng, M. Xia, F. Liang, D. Zhang, and X. Zhang Polymorphisms of Vasoactive Intestinal Peptide Receptor-1 Gene and Their Genetic Effects on Broodiness in Chickens Poult. Sci., May 1, 2008; 87(5): 893 - 903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Aguilera, E. Campos, R. Gimenez, J. Badia, J. Aguilar, and L. Baldoma Dual Role of LldR in Regulation of the lldPRD Operon, Involved in L-Lactate Metabolism in Escherichia coli J. Bacteriol., April 15, 2008; 190(8): 2997 - 3005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Eguchi, S. Kubota, K. Kawata, Y. Mukudai, J. Uehara, T. Ohgawara, S. Ibaragi, A. Sasaki, T. Kuboki, and M. Takigawa Novel Transcription Factor-Like Function of Human Matrix Metalloproteinase 3 Regulating the CTGF/CCN2 Gene Mol. Cell. Biol., April 1, 2008; 28(7): 2391 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Hashimi, G. Huang, A. Maxwell, and R. G. Birch DNA Gyrase from the Albicidin Producer Xanthomonas albilineans Has Multiple-Antibiotic-Resistance and Unusual Enzymatic Properties Antimicrob. Agents Chemother., April 1, 2008; 52(4): 1382 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Greiner, X. Wang, U. Rauwolf, M. V. Silber, K. Mayer, J. Meurer, G. Haberer, and R. G. Herrmann The complete nucleotide sequences of the five genetically distinct plastid genomes of Oenothera, subsection Oenothera: I. Sequence evaluation and plastome evolution Nucleic Acids Res., April 1, 2008; 36(7): 2366 - 2378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-M. Hsiao, M.-H. Zheng, R.-M. Hu, T.-C. Yang, and Y.-H. Tseng Regulation of the pehA gene encoding the major polygalacturonase of Xanthomonas campestris by Clp and RpfF Microbiology, March 1, 2008; 154(3): 705 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ramos, F. Lombo, A. F. Brana, J. Rohr, C. Mendez, and J. A. Salas Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster Microbiology, March 1, 2008; 154(3): 781 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. E. Rose, S. N. Leonard, G. Sakoulas, G. W. Kaatz, M. J. Zervos, A. Sheth, C. F. Carpenter, and M. J. Rybak Daptomycin Activity against Staphylococcus aureus following Vancomycin Exposure in an In Vitro Pharmacodynamic Model with Simulated Endocardial Vegetations Antimicrob. Agents Chemother., March 1, 2008; 52(3): 831 - 836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Wommack, J. Bhavsar, and J. Ravel Metagenomics: Read Length Matters Appl. Envir. Microbiol., March 1, 2008; 74(5): 1453 - 1463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. von Gromoff, A. Alawady, L. Meinecke, B. Grimm, and C. F. Beck Heme, a Plastid-Derived Regulator of Nuclear Gene Expression in Chlamydomonas PLANT CELL, March 1, 2008; 20(3): 552 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Belitsky and A. L. Sonenshein Genetic and Biochemical Analysis of CodY-Binding Sites in Bacillus subtilis J. Bacteriol., February 15, 2008; 190(4): 1224 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. White, M. Roberts, J. A. Yorke, and M. Pop Figaro: a novel statistical method for vector sequence removal Bioinformatics, February 15, 2008; 24(4): 462 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Qamar, P. M. Williams, and S. M. Lindsay Can an Atomic Force Microscope Sequence DNA Using a Nanopore? Biophys. J., February 15, 2008; 94(4): 1233 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. N. Bertin, C. Medigue, and P. Normand Advances in environmental genomics: towards an integrated view of micro-organisms and ecosystems Microbiology, February 1, 2008; 154(2): 347 - 359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fujimura and K. Murakami Staphylococcus aureus Clinical Isolate with High-Level Methicillin Resistance with an lytH Mutation Caused by IS1182 Insertion Antimicrob. Agents Chemother., February 1, 2008; 52(2): 643 - 647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Cuthbertson, Y. Liao, L. Birnbaumer, and P. J. Blackshear Characterization of zfs1 as an mRNA-binding and -destabilizing Protein in Schizosaccharomyces pombe J. Biol. Chem., February 1, 2008; 283(5): 2586 - 2594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matsuo, J. Chen, Y. Minato, W. Ogawa, T. Mizushima, T. Kuroda, and T. Tsuchiya SmdAB, a Heterodimeric ABC-Type Multidrug Efflux Pump, in Serratia marcescens J. Bacteriol., January 15, 2008; 190(2): 648 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. Fredlake, D. G. Hert, C.-W. Kan, T. N. Chiesl, B. E. Root, R. E. Forster, and A. E. Barron Ultrafast DNA sequencing on a microchip by a hybrid separation mechanism that gives 600 bases in 6.5 minutes PNAS, January 15, 2008; 105(2): 476 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. de Lencastre and A. M. Pyle Three essential and conserved regions of the group II intron are proximal to the 5'-splice site RNA, January 1, 2008; 14(1): 11 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fernandez, M. Sanchez-Hidalgo, N. Garcia-Quintans, M. Martinez-Bueno, E. Valdivia, P. Lopez, and M. Maqueda Processing of as-48ABC RNA in AS-48 Enterocin Production by Enterococcus faecalis J. Bacteriol., January 1, 2008; 190(1): 240 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Woolford, A. Rector, M. Van Ranst, A. Ducki, M. D. Bennett, P. K. Nicholls, K. S. Warren, R. A. Swan, G. E. Wilcox, and A. J. O'Hara A Novel Virus Detected in Papillomas and Carcinomas of the Endangered Western Barred Bandicoot (Perameles bougainville) Exhibits Genomic Features of both the Papillomaviridae and Polyomaviridae J. Virol., December 15, 2007; 81(24): 13280 - 13290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-G. Seo, S. W. Park, H. Park, S. Y. Kim, Y. T. Ro, E. Kim, J. W. Cho, and Y. M. Kim Cloning, characterization and expression of a gene encoding dihydroxyacetone synthase in Mycobacterium sp. strain JC1 DSM 3803 Microbiology, December 1, 2007; 153(12): 4174 - 4182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shinkai, N. Ohbayashi, T. Terada, M. Shirouzu, S. Kuramitsu, and S. Yokoyama Identification of Promoters Recognized by RNA Polymerase-{sigma}E Holoenzyme from Thermus thermophilus HB8 J. Bacteriol., December 1, 2007; 189(23): 8758 - 8764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Mandal, S. Gayen, A. Basu, K. S. Ghosh, S. Dasgupta, M. K. Maiti, and S. K. Sen Prediction-based protein engineering of domain I of Cry2A entomocidal toxin of Bacillus thuringiensis for the enhancement of toxicity against lepidopteran insects Protein Eng. Des. Sel., December 1, 2007; 20(12): 599 - 606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, T. W. Loo, M. C. Bartlett, and D. M. Clarke Correctors Promote Maturation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)-processing Mutants by Binding to the Protein J. Biol. Chem., November 16, 2007; 282(46): 33247 - 33251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kalyan Chakravarthy, T. N. R. Srinivas, P. Anil Kumar, Ch. Sasikala, and Ch. V. Ramana Roseospira visakhapatnamensis sp. nov. and Roseospira goensis sp. nov. Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2453 - 2457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Anil Kumar, T. S. Sasi Jyothsna, T. N. R. Srinivas, Ch. Sasikala, Ch. V. Ramana, and J. F. Imhoff Two novel species of marine phototrophic Gammaproteobacteria: Thiorhodococcus bheemlicus sp. nov. and Thiorhodococcus kakinadensis sp. nov. Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2458 - 2461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Brasme, P. Nordmann, F. Fidel, M. F. Lartigue, O. Bajolet, L. Poirel, D. Forte, V. Vernet-Garnier, J. Madoux, J. C. Reveil, et al. Incidence of class A extended-spectrum {beta}-lactamases in Champagne-Ardenne (France): a 1 year prospective study J. Antimicrob. Chemother., November 1, 2007; 60(5): 956 - 964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Luscher, P. Onal, A.-M. Schweingruber, and P. Maser Adenosine Kinase of Trypanosoma brucei and Its Role in Susceptibility to Adenosine Antimetabolites Antimicrob. Agents Chemother., November 1, 2007; 51(11): 3895 - 3901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Artin, P. Bjorkman, J. Cronqvist, P. Radstrom, and E. Holst First Case of Type E Wound Botulism Diagnosed Using Real-Time PCR J. Clin. Microbiol., November 1, 2007; 45(11): 3589 - 3594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Dohm, C. Lottaz, T. Borodina, and H. Himmelbauer SHARCGS, a fast and highly accurate short-read assembly algorithm for de novo genomic sequencing Genome Res., November 1, 2007; 17(11): 1697 - 1706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. B. Gavilano and B. Siminszky Isolation and Characterization of the Cytochrome P450 Gene CYP82E5v2 that Mediates Nicotine to Nornicotine Conversion in the Green Leaves of Tobacco Plant Cell Physiol., November 1, 2007; 48(11): 1567 - 1574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Jackson, K. R. Cronin, R. Zachariah, and J. A. Carew CCAAT/Enhancer-binding Protein-beta Participates in Insulin-responsive Expression of the Factor VII Gene J. Biol. Chem., October 26, 2007; 282(43): 31156 - 31165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wu, S. Zhang, Q. Meng, H. Cao, Z. Li, X. Li, S. Shi, D. H. Kim, L. Bi, N. J. Turro, et al. 3'-O-modified nucleotides as reversible terminators for pyrosequencing PNAS, October 16, 2007; 104(42): 16462 - 16467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xiong, C. E. Bauer, and A. Pancholy Insight into the haem d1 biosynthesis pathway in heliobacteria through bioinformatics analysis Microbiology, October 1, 2007; 153(10): 3548 - 3562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Hutchison III DNA sequencing: bench to bedside and beyond Nucleic Acids Res., September 25, 2007; 35(18): 6227 - 6237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Anil Kumar, T. N. R. Srinivas, Ch. Sasikala, and Ch. V. Ramana Halochromatium roseum sp. nov., a non-motile phototrophic gammaproteobacterium with gas vesicles, and emended description of the genus Halochromatium Int J Syst Evol Microbiol, September 1, 2007; 57(9): 2110 - 2113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. DeMarco, L. A. Cushing, E. Frempong-Manso, S. M. Seo, T. A. A. Jaravaza, and G. W. Kaatz Efflux-Related Resistance to Norfloxacin, Dyes, and Biocides in Bloodstream Isolates of Staphylococcus aureus Antimicrob. Agents Chemother., September 1, 2007; 51(9): 3235 - 3239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Iwase, J. Luo, E. Bi, and A. Toh-e Shs1 Plays Separable Roles in Septin Organization and Cytokinesis in Saccharomyces cerevisiae Genetics, September 1, 2007; 177(1): 215 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Takakura, H. Tsukamoto, and T. Yamamoto Molecular Cloning, Expression and Properties of an {alpha}/{beta}-Galactoside {alpha}2,3-Sialyltransferase from Vibrio sp. JT-FAJ-16 J. Biochem., September 1, 2007; 142(3): 403 - 412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Kleeb, M. H. Edalat, M. Gamper, J. Haugstetter, L. Giger, M. Neuenschwander, P. Kast, and D. Hilvert Metabolic engineering of a genetic selection system with tunable stringency PNAS, August 28, 2007; 104(35): 13907 - 13912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Jung, M. Hollmann, and M. A. Schafer The fatty acid elongase NOA is necessary for viability and has a somatic role in Drosophila sperm development J. Cell Sci., August 15, 2007; 120(16): 2924 - 2934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Wehrle, S. Renzullo, A. Faust, M. Beer, V. Kaden, and M. A. Hofmann Chimeric pestiviruses: candidates for live-attenuated classical swine fever marker vaccines J. Gen. Virol., August 1, 2007; 88(8): 2247 - 2258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Vilches, R. Canals, M. Wilhelms, M. T. Salo, Y. A. Knirel, E. Vinogradov, S. Merino, and J. M. Tomas Mesophilic Aeromonas UDP-glucose pyrophosphorylase (GalU) mutants show two types of lipopolysaccharide structures and reduced virulence Microbiology, August 1, 2007; 153(8): 2393 - 2404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Matveev, H. Nishihara, and N. Okada Novel SINE Families from Salmons Validate Parahucho (Salmonidae) as a Distinct Genus and Give Evidence that SINEs Can Incorporate LINE-related 3'-Tails of Other SINEs Mol. Biol. Evol., August 1, 2007; 24(8): 1656 - 1666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Garcel, J.-M. Crance, R. Drillien, D. Garin, and A.-L. Favier Genomic sequence of a clonal isolate of the vaccinia virus Lister strain employed for smallpox vaccination in France and its comparison to other orthopoxviruses J. Gen. Virol., July 1, 2007; 88(7): 1906 - 1916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Power, J. Di Conza, M. M. Rodriguez, B. Ghiglione, J. A. Ayala, J. M. Casellas, M. Radice, and G. Gutkind Biochemical Characterization of PER-2 and Genetic Environment of blaPER-2 Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2359 - 2365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. V. Thijs, S. C. J. De Keersmaecker, A. Fadda, K. Engelen, H. Zhao, M. McClelland, K. Marchal, and J. Vanderleyden Delineation of the Salmonella enterica Serovar Typhimurium HilA Regulon through Genome-Wide Location and Transcript Analysis J. Bacteriol., July 1, 2007; 189(13): 4587 - 4596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Grassadonia, N. Tinari, B. Fiorentino, M. Nakazato, H.-K. Chung, C. Giuliani, G. Napolitano, S. Iacobelli, T. K. Howcroft, D. S. Singer, et al. Upstream Stimulatory Factor Regulates Constitutive Expression and Hormonal Suppression of the 90K (Mac-2BP) Protein Endocrinology, July 1, 2007; 148(7): 3507 - 3517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. Perkins, M. Taniguchi, D. O. Warner, E. N. Chini, and K. A. Jones Reduction in soluble guanylyl cyclase-specific activity following prolonged treatment of porcine pulmonary artery with nitric oxide Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L84 - L95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sugiura, M. N. Georgescu, and M. Takahashi A Nitrite Transporter Associated with Nitrite Uptake by Higher Plant Chloroplasts Plant Cell Physiol., July 1, 2007; 48(7): 1022 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Sen, C. T. Huang, K. Han, and M. A. Batzer Endonuclease-independent insertion provides an alternative pathway for L1 retrotransposition in the human genome Nucleic Acids Res., June 28, 2007; 35(11): 3741 - 3751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ochiai, T. Itoh, A. Kawamata, W. Hashimoto, and K. Murata Plant Cell Wall Degradation by Saprophytic Bacillus subtilis Strains: Gene Clusters Responsible for Rhamnogalacturonan Depolymerization Appl. Envir. Microbiol., June 15, 2007; 73(12): 3803 - 3813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
