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Vol. 93, Issue 12, 5925-5930, June 11, 1996
* Center for Biotechnology and ¶ Department of Medical
Nutrition, Karolinska Institute, NOVUM, S-14186 Huddinge, Sweden;
Communicated by Elwood V. Jensen, IHF Institute for Hormone and
Fertility Research, Hamburg, Germany, February 13, 1996 (received for review October 30, 1995)
We have cloned a novel member of the nuclear receptor superfamily.
The cDNA of clone 29 was isolated from a rat prostate cDNA library and
it encodes a protein of 485 amino acid residues with a calculated
molecular weight of 54.2 kDa. Clone 29 protein is unique in that it is
highly homologous to the rat estrogen receptor (ER) protein,
particularly in the DNA-binding domain (95%) and in the C-terminal
ligand-binding domain (55%). Expression of clone 29 in rat tissues was
investigated by in situ hybridization and prominent
expression was found in prostate and ovary. In the prostate clone 29 is
expressed in the epithelial cells of the secretory alveoli, whereas in
the ovary the granulosa cells in primary, secondary, and mature
follicles showed expression of clone 29. Saturation ligand-binding
analysis of in vitro synthesized clone 29 protein revealed
a single binding component for 17
0027-8424/96/935925-6/0
Biochemistry
Cloning of a novel estrogen receptor expressed in rat prostate
and ovary
,§,
, and
KaroBio AB, NOVUM, S-14157 Huddinge, Sweden;
Department of Neurology, University of Kuopio, Kuopio, Finland;
and § Department of Anatomy, Tampere University Medical
School, P.O. Box 607, FIN-33101 Tampere, Finland
-estradiol (E2) with high affinity
(Kd= 0.6 nM). In ligand-competition experiments the binding affinity decreased in the order E2 > diethylstilbestrol > estriol > estrone > 5
-androstane-3,17-diol 
testosterone = progesterone = corticosterone = 5-androstane-3,17-diol. In cotransfection experiments of
Chinese hamster ovary cells with a clone 29 expression vector and an
estrogen-regulated reporter gene, maximal stimulation (about 3-fold) of
reporter gene activity was found during incubation with 10 nM of E2.
Neither progesterone, testosterone, dexamethasone, thyroid hormone,
all-trans-retinoic acid, nor 5-androstane-3,17-diol could
stimulate reporter gene activity, whereas estrone and
5-androstane-3,17-diol did. We conclude that clone 29 cDNA
encodes a novel rat ER, which we suggest be named rat ER to
distinguish it from the previously cloned ER (ER) from rat uterus.
To whom reprint requests should be addressed. e-mail:
Jan-Ake.Gustafsson{at}cnt.ki.se.
CiteULike 
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