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Vol. 93, Issue 12, 6025-6030, June 11, 1996

Biochemistry
 Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries

Luda Diatchenko*, Yun-Fai Chris Laudagger , Aaron P. Campbelldagger , Alex Chenchik*, Fauzia Moqadam*, Betty Huang*, Sergey LukyanovDagger , Konstantin LukyanovDagger , Nadya GurskayaDagger , Eugene D. SverdlovDagger , and Paul D. Siebert*

* CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303; dagger  Division of Cell and Developmental Genetics, Department of Medicine, University of California, San Francisco, CA 94121; and Dagger  Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Mikluho-Maklaya 16/10, V-437 Moscow 117871, Russia

Communicated by Mark M. Davis, Stanford University School of Medicine, Stanford, CA, February 1, 1996 (received for review October 26, 1995)

A new and highly effective method, termed suppression subtractive hybridization (SSH), has been developed for the generation of subtracted cDNA libraries. It is based primarily on a recently described technique called suppression PCR and combines normalization and subtraction in a single procedure. The normalization step equalizes the abundance of cDNAs within the target population and the subtraction step excludes the common sequences between the target and driver populations. In a model system, the SSH technique enriched for rare sequences over 1,000-fold in one round of subtractive hybridization. We demonstrate its usefulness by generating a testis-specific cDNA library and by using the subtracted cDNA mixture as a hybridization probe to identify homologous sequences in a human Y chromosome cosmid library. The human DNA inserts in the isolated cosmids were further confirmed to be expressed in a testis-specific manner. These results suggest that the SSH technique is applicable to many molecular genetic and positional cloning studies for the identification of disease, developmental, tissue-specific, or other differentially expressed genes.

0027-8424/96/936025-6/0
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