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* Columbia Genome Center, Columbia University College of Physicians
and Surgeons, New York, NY 10032; § Department of Pathology,
Columbia University College of Physicians and Surgeons, New York, NY
10032; and ¶ Division of Epidemiology, School of Public Health,
Columbia University, New York, NY 10032
Contributed by Isidore S. Edelman, October 7, 1996
The genome of the Kaposi sarcoma-associated herpesvirus (KSHV or
HHV8) was mapped with cosmid and phage genomic libraries from the BC-1
cell line. Its nucleotide sequence was determined except for a 3-kb
region at the right end of the genome that was refractory to cloning.
The BC-1 KSHV genome consists of a 140.5-kb-long unique coding region
flanked by multiple G+C-rich 801-bp terminal repeat sequences. A
genomic duplication that apparently arose in the parental tumor is
present in this cell culture-derived strain. At least 81 ORFs,
including 66 with homology to herpesvirus saimiri ORFs, and 5 internal
repeat regions are present in the long unique region. The virus encodes
homologs to complement-binding proteins, three cytokines (two
macrophage inflammatory proteins and interleukin 6), dihydrofolate
reductase, bcl-2, interferon regulatory factors,
interleukin 8 receptor, neural cell adhesion molecule-like adhesin, and
a D-type cyclin, as well as viral structural and metabolic proteins.
Terminal repeat analysis of virus DNA from a KS lesion suggests a
monoclonal expansion of KSHV in the KS tumor.
Proc. Natl. Acad. Sci. USA
Vol. 93,
pp. 14862-14867,
December 1996
Microbiology
Nucleotide sequence of the Kaposi
sarcoma-associated herpesvirus (HHV8)
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