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Proc. Natl. Acad. Sci. USA
Vol. 93, pp. 14862-14867, December 1996
Microbiology

Nucleotide sequence of the Kaposi sarcoma-associated herpesvirus (HHV8)

James J. Russo*, Roy A. Bohenzky§, Ming-Cheng Chien*, Jing Chen*, Ming Yan§, Dawn Maddalena§, J. Preston Parry, Daniela Peruzzi§, Isidore S. Edelman*, Yuan Chang§, and Patrick S. Moore

* Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032; § Department of Pathology, Columbia University College of Physicians and Surgeons, New York, NY 10032; and  Division of Epidemiology, School of Public Health, Columbia University, New York, NY 10032

Contributed by Isidore S. Edelman, October 7, 1996

The genome of the Kaposi sarcoma-associated herpesvirus (KSHV or HHV8) was mapped with cosmid and phage genomic libraries from the BC-1 cell line. Its nucleotide sequence was determined except for a 3-kb region at the right end of the genome that was refractory to cloning. The BC-1 KSHV genome consists of a 140.5-kb-long unique coding region flanked by multiple G+C-rich 801-bp terminal repeat sequences. A genomic duplication that apparently arose in the parental tumor is present in this cell culture-derived strain. At least 81 ORFs, including 66 with homology to herpesvirus saimiri ORFs, and 5 internal repeat regions are present in the long unique region. The virus encodes homologs to complement-binding proteins, three cytokines (two macrophage inflammatory proteins and interleukin 6), dihydrofolate reductase, bcl-2, interferon regulatory factors, interleukin 8 receptor, neural cell adhesion molecule-like adhesin, and a D-type cyclin, as well as viral structural and metabolic proteins. Terminal repeat analysis of virus DNA from a KS lesion suggests a monoclonal expansion of KSHV in the KS tumor.


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