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Vol. 95, Issue 24, 14250-14255, November 24, 1998

Evolution
A heterodimeric DNA polymerase: Evidence that members of Euryarchaeota possess a distinct DNA polymerase

(Archaea/DNA replication/DNA Polymerase /hyperthermophile/methanogen)

Isaac K. O. Cann^*, Kayoko Komori^*, Hiroyuki Toh, Satoru Kanai, and Yoshizumi Ishino^*,

Departments of ^* Molecular Biology and  Bioinformatics, Biomolecular Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565, Japan

Communicated by Carl R. Woese, University of Illinois at Urbana-Champaign, Urbana, IL, August 6, 1998 (received for review April 29, 1998)

We describe here a DNA polymerase family highly conserved in Euryarchaeota, a subdomain of Archaea. The DNA polymerase is composed of two proteins, DP1 and DP2. Sequence analysis showed that considerable similarity exists between DP1 and the second subunit of eukaryotic DNA polymerase , a protein essential for the propagation of Eukarya, and that DP2 has conserved motifs found in proteins with nucleotide-polymerizing activity. These results, together with our previous biochemical analyses of one of the members, DNA polymerase II (DP1 + DP2) from Pyrococcus furiosus, implicate the DNA polymerases of this family in the DNA replication process of Euryarchaeota. The discovery of this DNA-polymerase family, aside from providing an opportunity to enhance our knowledge of the evolution of DNA polymerases, is a significant step toward the complete understanding of DNA replication across the three domains of life.

Copyright © 1998 by The National Academy of Sciences  0027-8424/98/9514250-6$2.00/0
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