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Vol. 95, Issue 7, 3396-3401, March 31, 1998

Biochemistry
 Linker histone protects linker DNA on only one side of the core particle and in a sequence-dependent manner

Woojin An*, Sanford H. Leuba*,dagger , Kensal van Holde*, and Jordanka Zlatanova*,§

* Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331-7305; and dagger  Institute of Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

Contributed by Kensal van Holde, January 20, 1998

The protection against micrococcal nuclease digestion afforded to chromatosomal DNA by the presence of a linker histone (H1o) has been quantitatively measured in two reconstituted systems. We have used chromatosomes reconstituted at two distinct positions on a DNA fragment containing the 5S rRNA gene from Lytechinus variegatus and at a specific position on a sequence containing Gal4- and USF-binding sites. In all cases, we find asymmetric protection, with approx 20 bp protected on one side of the core particle and no protection on the other. We demonstrated through crosslinking experiments that the result is not due to any sliding of the histone core caused by either linker histone addition or micrococcal nuclease cleavage. Because the core particle is itself a symmetric object, the preferred asymmetric location of a linker histone must be dictated by unknown elements in the DNA sequence.

dagger    Present address: Department of Physics and Astronomy, Arizona State University, P.O. Box 871504, Tempe, AZ 85287-1504.
§   To whom reprint requests should be addressed at: Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331-7305. e-mail: zlatanoj{at}ucs.orst.edu.

Copyright © 1998 by The National Academy of Sciences  0027-8424/98/953396-6$2.00/0
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