Alteration in the gene expression pattern of primary monocytes after adhesion to endothelial cells

Thomas-Ecker et al.10.1073/pnas.0700732104.

Supporting Information

Files in this Data Supplement:

SI Table 2
SI Table 3




Table 2. Gene regulation in monocytes after adhesion to endothelial cells

Accession no./clone ID

Gene

Fold change

Predominant expression

M15330

interleukin 1-beta (IL-1B)

14.139

phagocyte lineage

M13509

matrix metalloproteinase-1 (MMP-1)

8.676

phagocyte lineage

M98479

tissue transglutaminase-2

5.830

phagocyte lineage

M27024

LPS associated protein 2

5.166

phagocyte lineage

M24545

monocyte chemoattractane protein-1 (MCP-1)

5.013

phagocyte lineage

M13560

CD74 invariant chain

5.013

phagocyte lineage

X06820

rhoB

4.810

phagocyte lineage

Z18951

caveolin-1

4.728

phagocyte lineage

X15606

ICAM-2, cell adhesion ligand for LFA-1

3.321

phagocyte lineage

M63835

IgG Fc receptor I (CD64)

3.021

phagocyte lineage

IMAGE:127269

chemokine (C-C motif) ligand 15

2.745

phagocyte lineage

J03225

tissue factor pathway inhibitor

2.550

phagocyte lineage

X68277

dual specificity phosphatase-1

2.527

phagocyte lineage

V01512

cellular oncogene c-fos

2.323

phagocyte lineage

L08187

Epstein-Barr virus-induced gene 3 (EBI3)

0.426

phagocyte lineage

Z36714

caspase 1#

0.236

phagocyte lineage

X53331

Matrix Gla protein

27.523

mesenchymal lineage

IMAGE:154494

insulin-like growth factor binding protein-4 (IGFBP4)

16.057

mesenchymal lineage

X83703

ankyrin repeart domain 1 (cardiac muscle)

8.415

mesenchymal lineage

M30640

E-selectin

7.439

mesenchymal lineage

X78947

Connective tissue growth factor (CTGF)

6.361

mesenchymal lineage

IMAGE:44477

vascular cell adhesion molecule-1 (VCAM1/ CD106)

5.895

mesenchymal lineage

X02761

fibronectin-1

4.035

mesenchymal lineage

W73702

aggrecanase-2

3.157

mesenchymal lineage

AF043045

actin-binding protein ABP-278 (filamin B, beta)

2.406

mesenchymal lineage

M57730

ephrin-A1

2.104

mesenchymal lineage

J02973

thrombomodulin

2.035

mesenchymal lineage

X07173

inter-alpha (globulin) inhibitor, H2 polypeptide

0.362

mesenchymal lineage

AF015043

SH3-domain binding protein

0.080

mesenchymal lineage

AF039688

hemoglobin, alpha 2

0.183

hematopoietic lineage

M25897

platelet factor 4

0.168

hematopoietic lineage

M54995

pro-platelet basic protein (CTAP-3)

0.155

hematopoietic lineage

X01388

hemoglobin, gamma G (HBG2)#

0.144

hematopoietic lineage

X02747

hemoglobin, beta#

0.094

hematopoietic lineage

L48218

hemoglobin, beta

0.088

hematopoietic lineage

U90544

hemoglobin, gamma G (HBG2)#

0.084

hematopoietic lineage

U19718

microfibrillar-associated glycoprotein (MFAP2)

6.847

misc

IMAGE:32319

Ubiquitin carboxyl-terminal esterase L1

4.746

misc

AF111168

serine palmitoyl transferase

4.359

misc

AB016811

ADP-ribosylation factor-like 7

4.188

misc

X54380

pregnancy zone protein

3.934

misc

D87735

ribosomal protein L14

3.794

misc

AB007926

disrupted in schizophrenia 1

3.033

misc

AF093265

homer-3

2.586

misc

IMAGE:44198

paraneoplastic neuronal antigen MA1 (MA1)

2.310

misc

AF039023

Ran-GTP binding protein

2.280

misc

30827

oncoprotein 18 (Op18)

2.012

misc

U89942

lysyl oxidase like 2 (LOXL2)

0.496

misc

U90268

krit1

0.472

misc

AF071510

lecithin retinol acyltransferase (LRAT)

0.462

misc

U62767

dihydrouridine synthase 4-like

0.412

misc

U58331

sarcoglycan, delta

0.357

misc

U90313

glutathione-S-transferase omaga-1

0.284

misc

S79854

deiodinase, iodothyronine, type III

0.236

misc

X03557

interferon-induced protein with tetratricopeptide repeats 1

0.229

misc

L23134

granzyme M

0.185

misc

Genes were grouped according to known predominant tissue expression including phagocyte lineage, mesenchymal lineage, and hematopoietic lineage. Genes with unknown function or which could not be associated with a certain tissue were designated as "misc." All significantly regulated genes are displayed, and the shown fold change represents the mean of five independent experiments. Genes marked with "#" were renamed after gene sequencing.




Table 3. Gene regulation in monocytes after adhesion to plastic

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Accession no./clone ID

Gene

Fold change

Predominant expression

AA866071

Wilms tumor associated protein

3.449

phagocyte lineage

D84109

RNA-binding protein gene with multiple splicing

2.553

phagocyte lineage

AA046832

S100 calcium binding protein, beta (neural)

2.239

phagocyte lineage

U09413

Zinc finger protein 135

2.148

phagocyte lineage

AF039291

lysyl oxidase

2.114

phagocyte lineage

U91521

peroxisomal biogenesis factor 12

2.101

phagocyte lineage

M86757

S100 calcium binding protein A7 (psoriasin 1)

2.025

phagocyte lineage

M59371

EphA2

2.011

phagocyte lineage

Z36531

fibrinogen-like 2

0.445

phagocyte lineage

X13238

cytochrome c oxidase subunit VIc

0.433

phagocyte lineage

U00672

interleukin 10 receptor, alpha

0.433

phagocyte lineage

U20734

Jun B

0.431

phagocyte lineage

AF078077

growth arrest and DNA-damage-inducible, beta

0.422

phagocyte lineage

U55766

HIV-1 rev binding protein 2

0.419

phagocyte lineage

X68277

dual specificity phosphatase 1

0.409

phagocyte lineage

D31767

DAZ associated protein 2

0.407

phagocyte lineage

M15395

integrin, beta 2 (antigen CD18).

0.391

phagocyte lineage

M92357

tumor necrosis factor alpha-induced protein 2

0.390

phagocyte lineage

X16832

cathepsin H

0.383

phagocyte lineage

X13621

defensin, alpha 3, neutrophil-specific

0.370

phagocyte lineage

M98398

CD36 antigen (collagen type I receptor, thrombospondin receptor)

0.368

phagocyte lineage

U15085

major histocompatibility complex class II DM beta

0.366

phagocyte lineage

Z22576

CD69 antigen

0.352

phagocyte lineage

X15998

chondroitin sulfate proteoglycan 2 (versican)

0.343

phagocyte lineage

M81750

myeloid cell nuclear differentiation antigen

0.296

phagocyte lineage

X81789

splicing factor 3a, subunit 3, 60kD

0.214

phagocyte lineage

M54995

pro-platelet basic protein (CTAP-3)

0.271

hematopoietic lineage

L10347

collagen type II alpha 1

3.697

misc

U40992

DnaJ (Hsp40) homolog, subfamily B, member 4

2.403

misc

AB018351

thymus high mobility group box protein TOX

2.385

misc

AB018566

proline synthetase co-transcribed homolog (bacterial)

2.201

misc

AF043472

potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3

2.140

misc

AI023703

amyloid P component, serum

2.137

misc

AF042181

TSPY-like

2.122

misc

M93107

3-hydroxybutyrate dehydrogenase (heart. mitochondrial)

2.044

misc

Z12830

signal sequence receptor, alpha

0.449

misc

M31470

ras-like protein TC10

0.448

misc

M97191

Sp3 transcription factor

0.448

misc

AJ001875

potassium inwardly-rectifying channel, subfamily J, member 6

0.448

misc

U36336

lysosomal-associated membrane protein 2

0.447

misc

X52839

ribosomal protein L23

0.443

misc

AB006623

transmembrane protein 24

0.441

misc

346735

up-regulated gene 4

0.440

misc

AF035191

nuclear autoantigenic sperm protein (histone-binding)

0.438

misc

135381

protein phosphatase 1, regulatory (inhibitor) subunit 15A

0.436

misc

S79639

exostoses (multiple) 1

0.432

misc

L25081

ras homolog gene family, member C

0.431

misc

AJ005890

coiled-coil domain containing 22

0.431

misc

AF009923

cathepsin Z

0.431

misc

152466

phosphogluconate dehydrogenase

0.422

misc

X08094

glutathione S-transferase pi

0.417

misc

U61263

ilvB (bacterial acetolactate synthase)-like

0.416

misc

X06956

alpha-tubulin 4

0.414

misc

M14539

coagulation factor XIII, A1 polypeptide

0.413

misc

M99487

folate hydrolase (prostate-specific membrane antigen) 1

0.408

misc

X79568