Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance

doi:10.1073/pnas.0703758104

Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance

Departments of *Crop and Soil Sciences and
^¶Plant Pathology, U.S. Department of Agriculture–Agricultural Research Service and
^‡School of Molecular Biosciences, Washington State University, Pullman, WA 99164; and
^†Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108

Contributed by Diter von Wettstein, April 25, 2007 (received for review February 4, 2007)

Abstract

In plants, disease resistance mediated by the gene-for-gene mechanism involves the recognition of specific effector molecules produced by the pathogen either directly or indirectly by the resistance-gene products. This recognition triggers a series of signals, thereby serving as a molecular switch in regulating defense mechanisms by the plants. To understand the mechanism of action of the barley stem rust resistance gene Rpg1, we investigated the fate of the RPG1 protein in response to infection with the stem rust fungus, Puccinia graminis f. sp. tritici. The investigations revealed that RPG1 disappears to undetectable limits only in the infected tissues in response to avirulent, but not virulent pathotypes. The RPG1 protein disappearance is rapid and appears to be due to specific protein degradation via the proteasome-mediated pathway as indicated by inhibition with the proteasomal inhibitor MG132, but not by other protease inhibitors.

Footnotes

^§To whom correspondence should be addressed. E-mail: diter{at}wsu.edu
Author contributions: J.N., B.J.S., and A.K. designed research; J.N., S.D., and B.J.S. performed research; C.G.K. contributed new reagents/analytic tools; D.v.W., X.C., and A.K. analyzed data; and J.N., D.v.W., and A.K. wrote the paper.
The authors declare no conflict of interest.
This article contains supporting information online at www.pnas.org/cgi/content/full/0703758104/DC1.
Abbreviations:

Avr,

avirulence;

cv.,

cultivar;

hai,

hours after infection;

R,

resistance;

PCD,

programmed cell death;

Pgt,

Puccinia graminis f. sp. tritici;

Pgs,

Puccinia graminis f. sp. secalis.