IFN-stimulated gene 15 functions as a critical antiviral molecule against influenza, herpes, and Sindbis viruses

Lenschow et al. 10.1073/pnas.0607038104.

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Fig. 5. No alterations in growth of herpes or Sindbis viruses in ISG15-^/- murine embryonic fibroblasts (MEFs). ISG15^+/+ (open squares) and ISG15-^/- (closed squares) MEFs were infected with herpes simplex virus 1 (HSV-1) (A) or gammaherpesvirus 68 (gHV68) (B) at a multiplicity of infection (moi) of 0.05 or with dsTE12Q (C) at an moi of 5.0, and samples were harvested at the indicated time points to assay for growth by plaque assay. Data represent two independent experiments. Error bars represent SEM.

SI Figure 6

Fig. 6. Identification of ISG15 in the sera of mice after viral infection. (A) Sera were isolated from 129 mice 3 days after treatment with LPS (20 mg i.p.), pI:pC (100 mg i.p.), or infection with gHV68 (1 ´ 10⁶ pfu i.p.) and analyzed for the presence of ISG15 by ELISA. (B) Sera were isolated from ISG15^+/+ or ISG15- ^/- mice that were mock-infected or infected with influenza B/Lee virus (1 ´ 10⁶ pfu intranasally) for 6 days. In both A and B, ISG15 was detected in the sera by capture ELISA (using previously characterized polyclonal and mAbs to mISG15; ref. 21). Recombinant 15-kDa ISG15 was used as the standard control. (C) ISG15 was detected by Western blot analysis with anti-ISG15 mAb (3C2) in the sera isolated from 129 mice 3 days after infection with gHV68. Mass spectroscopy analysis of the 15-kDa band confirmed the presence of ISG15.