An infectious retrovirus susceptible to an IFN antiviral pathway from human prostate tumors
- Beihua Dong*,
- Sanggu Kim†,
- Seunghee Hong*,‡,
- Jaydip Das Gupta*,
- Krishnamurthy Malathi*,
- Eric A. Klein§,
- Don Ganem¶,‖,**,
- Joseph L. DeRisi**,††,
- Samson A. Chow†,‡‡, and
- Robert H. Silverman*,§§
- *Department of Cancer Biology, Lerner Research Institute, and
- §Glickman Urologic Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195;
- †Biomedical Engineering Interdepartmental Program, University of California, Los Angeles, CA 90095;
- ‡Graduate Program in Molecular Virology, Case Western Reserve University, Cleveland, OH 44106;
- Departments of ††Biochemistry and Biophysics,
- ¶Microbiology, and
- ‖Medicine and
- **Howard Hughes Medical Institute, University of California, San Francisco, CA 94158; and
- ‡‡Department of Molecular and Medical Pharmacology, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095
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Communicated by George R. Stark, Cleveland Clinic Foundation, Cleveland, OH, November 27, 2006 (received for review November 6, 2006)
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Fig. 1.Cloning of full-length, replication-competent XMRV strain VP62. (A) Cloning strategy for assembling complete XMRV molecular viral clone VP62. The cloning diagram is aligned to the gene map. (B) Radiolabeled RT products from CM of LNCaP cells transfected for 10 days with 4 μg (lanes 1 and 2) and 2 μg (lanes 3 and 4) of VP62/pcDNA3.1 or from 4 μg of empty vector pcDNA3.1 (lane 5). (C Left) RT products in CM from DU145 cells previously exposed to 100 μl of CM from LNCaP cells transfected with pcDNA3.1 (lane 1) or to 1 μl (lane 2), 10 μl (lane 3), and 100 μl (lane 4) of CM from LNCaP cells transfected with VP62/pcDNA3.1. (C Right) Western blot for Gag and β-actin from DU145 cells incubated for 9 days with 100 μl of CM from pcDNA3.1-transfected LNCaP cells (lane 1) or CM from VP62/pcDNA3.1-transfected LNCaP cells. Lane 2, 10 μl of CM; lane 3, 100 μl of CM.
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Fig. 2.IFN sensitivity of XMRV in DU145 and LNCaP cells. (A and B) DU145 cells (A) or LNCaP cells (B) plated and assayed in triplicate were incubated for 16 h in the absence or presence of different amounts of IFN-β as indicated and then mock-infected (lane 1) or infected with XMRV (lanes 2–6) for 3 days. IFN-β was added a second time at 24 h after infection. (Upper) Autoradiograms of the radiolabeled RT products. (Lower) RT activity (cpm) as a function of [IFN-β]. (C) Effect of time of IFN-β treatment on viral yields in DU145 cells. The IFN added at −24 h only was removed and not replaced at the time of infection. Assays were performed in triplicate. The decreases in RT activity in response to IFN treatments were significant. P = 0.014 and 0.018 in two-tailed, paired Student's t tests at 20 and 2,000 units/ml IFN-β in A and B, respectively.
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Fig. 3.Effect of RNase L on the antiviral activity of IFN-β. (A and B Top) RT activities from CM of DU145 cells expressing short hairpin RNA to RNase L (siRNL) or expressing a three-base mismatch control RNA (siRNLm3) (as indicated). Cells were infected for 12 days with XMRV before addition of IFN-β. Lanes 1 and 7, media control; lanes 2–6 and 8–12, CM after 3 days of IFN treatment. (B Middle and Bottom) Western blots for RNase L and β-actin were from the same experiment, blot, and exposure. Two-tailed, paired Student's t tests were performed in B.
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Fig. 4.Infection of hamster cells with XMRV depends on expression of human XPR1. (A) Diagram of quantitative real-time RT-PCR strategy for amplifying an 84-bp region from the 5′ UTR of XMRV gag (nucleotides 445–528). (B) XMRV RNA copy number in CHO cells transiently transfected or mock-infected with empty vector pcDNA3.1 or human XPR1 cDNA in vector pcDNA3.1 followed by exposure to XMRV and continuous culturing for 30 days. The experiment was performed in triplicate. (C) Nested RT-PCR for XPR1, XMRV gag, and GAPDH RNAs in a representative experiment from B. An agarose gel in which the PCR products were stained with ethidium bromide is shown.
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Fig. 5.Locations of XMRV integration sites in prostate DNA from case VP268 (A and B) and case VP234 (C). Genomic DNA was isolated from the patient tumor sample, and the DNA sequence near the virus–host DNA junction was cloned and sequenced (Materials and Methods). (A) In chromosome 7p15.1 the integrated provirus was 2,640 bp upstream of the CREB5 transcription start site. (B) In chromosome 16q22.1 the integrated provirus was 1,816 bp downstream of the NFATc3 transcription start site. (C) In chromosome 17q23.2 the integrated provirus was 11,888 bp downstream of the APPBP2 transcription start site. Lowercase letters represent the sequence at the U5 end of the viral long terminal repeat, and uppercase letters represent human genomic sequences. Arrows denote the virus–host DNA junctions. Right-angled arrows denote the transcription start sites.
Footnotes
- §§To whom correspondence should be addressed. E-mail: silverr{at}ccf.org
- © 2007 by The National Academy of Sciences of the USA
