Stimulation of endogenous neurogenesis by anti-EFRH immunization in a transgenic mouse model of Alzheimer's disease

Becker et al. 10.1073/pnas.0610180104.

Supporting Information

Files in this Data Supplement:

Figure 5



Fig. 5. Anti-EFRH immunization effect on amyloid burden (I), astrocytic activation (II), microglial activation (III). Four-month-old PDAPP Tg mice (n = 12) were immunized i.p. with MAP-EFRH, emulsified in complete or incomplete Freund's adjuvant, five times, once every 2 weeks. Two additional boosts were injected at 12 and 13 months of age. (Ia) Brain sections of PDAPP Tg control and Tg treated mice stained with thioflavine S (Thio-S). (Iaa and Iac) PDAPP Tg control mice with amyloid burden of 2.0% exhibiting many plaques. (Iab and Iad) PDAPP Tg treated animal, with amyloid burden of 0.07% exhibiting only few plaques. (Iaa-Iad) Cortex (Iaa and Iab) and hippocampus (Iac and Iad) at the level of -1.6 from bregma. (Ib) Amyloid burden calculated as percentage of measured amyloid plaque area stained by Thio-S of the total section area. The results are presented as the mean of amyloid burden of two sections -1.6 and -3.8 from bregma of the right hemisphere. Results show a significant reduction of amyloid burden in Tg treated vs. Tg control mice. *, P £ 0.0006 compared with Tg control mice. (IIa) Brain sections of Tg control and Tg treated mice were stained with rabbit a-GFAP antibody. In Tg control mice (Left) the GFAP labeled astrocytes were widely distributed in areas of white and gray matter such as internal (a) and external (b) capsule; corpus callosum (c), hippocampus (d), and fimbria (e), cortex (f), amygdala (g), and hypothalamus (h). In PDAPP Tg treated mice (Right) the GFAP load was reduced. (IIb) GFAP load was presented as percentage of measured GFAP labeled area of the total section area. The results presented as a mean of GFAP load of two sections -1.6 and -3.8 from bregma. Significant reduction in GFAP load was measured in Tg treated mice vs. Tg control mice. (* P £ 0.032). (IIIa) Brain sections of Tg control and treated mice were stained with rat a-F4/80 antibody. F4/80 labeled ramified microglia were widely distributed throughout the brain in Tg control (IIIaa) and Tg treated (IIIab) mice (bar = 50 mm). (IIIb) F4/80 labeling scores show slight decrease in F4/80 labeling in the Tg treated compared to Tg control untreated animals, without significant statistical difference.

This Article

  1. Published online before print January 22, 2007, doi: 10.1073/pnas.0610180104 PNAS January 30, 2007 vol. 104 no. 5 1691-1696
  1. AbstractFree
  2. Figures Only
  3. Full Text
  4. Full Text (PDF)
  5. » Supporting Figure
About Our New Site Design >>
Link: Advanced Search

This Week's Issue

  1. August 19, 2008, 105 (33)
  1. Current Issue
  1. Alert me to new issues of PNAS

Most