## New Research In

### Physical Sciences

### Social Sciences

#### Featured Portals

#### Articles by Topic

### Biological Sciences

#### Featured Portals

#### Articles by Topic

- Agricultural Sciences
- Anthropology
- Applied Biological Sciences
- Biochemistry
- Biophysics and Computational Biology
- Cell Biology
- Developmental Biology
- Ecology
- Environmental Sciences
- Evolution
- Genetics
- Immunology and Inflammation
- Medical Sciences
- Microbiology
- Neuroscience
- Pharmacology
- Physiology
- Plant Biology
- Population Biology
- Psychological and Cognitive Sciences
- Sustainability Science
- Systems Biology

# Independent filtering increases detection power for high-throughput experiments

Edited by Stephen E. Fienberg, Carnegie Mellon University, Pittsburgh, PA, and approved March 22, 2010 (received for review December 3, 2009)

## Abstract

With high-dimensional data, variable-by-variable statistical testing is often used to select variables whose behavior differs across conditions. Such an approach requires adjustment for multiple testing, which can result in low statistical power. A two-stage approach that first filters variables by a criterion independent of the test statistic, and then only tests variables which pass the filter, can provide higher power. We show that use of some filter/test statistics pairs presented in the literature may, however, lead to loss of type I error control. We describe other pairs which avoid this problem. In an application to microarray data, we found that gene-by-gene filtering by overall variance followed by a *t*-test increased the number of discoveries by 50%. We also show that this particular statistic pair induces a lower bound on fold-change among the set of discoveries. Independent filtering—using filter/test pairs that are independent under the null hypothesis but correlated under the alternative—is a general approach that can substantially increase the efficiency of experiments.

In many experimental contexts which generate high-dimensional data, variable-by-variable statistical testing is used to select variables whose behavior differs across the set of studied conditions. Each variable is associated with a null hypothesis which asserts that behavior for that variable does not differ across conditions. A null hypothesis is rejected when observed data, summarized into a per-variable *p*-value, are deemed to be inconsistent with the hypothesis. In biology, for example, microarrays or high-throughput sequencing may be used to identify genes (variables) whose expression level shows systematic covariation with a treatment or phenotype of interest. The evidence for such covariation is assessed by applying a statistical test to each gene separately. In the case of microarrays, gene-by-gene *t*-tests are frequently used for two-class comparisons. This approach can be generalized to more complex experimental designs through the use of ANOVA (1); it has also been refined for experiments with small sample sizes by the introduction of moderated variance estimators (2), as in the *SAM* (3) and *limma* (4) software. When transcript abundance is measured by high-throughput sequencing rather than microarrays, gene-level *p*-values may instead be computed on the basis of gene-level read count statistics (5).

Because a large number of hypothesis tests are performed in such variable-by-variable analyses, many true-null hypotheses will produce small *p*-values by chance. As a consequence, numerous false positives, or type I errors, will result if *p*-values are compared to standard single-test thresholds. There are well-established procedures which address the multiple testing problem by adjusting the *p*-values to control various experiment-wide false positive measures, e.g., the family-wise error rate (FWER) or the false discovery rate (FDR). (See ref. 6 for a review).

Multiple testing adjustment provides control over the extent to which false positives occur, but such control comes at the cost of reduced power to detect true positives. Further, this power reduction worsens as more hypotheses are tested. Typically, the number of genes represented on a microarray is in the tens of thousands, while the number of differentially expressed genes may be only a few dozen or hundred. As a consequence, the power of an experiment to detect a given differentially expressed gene could potentially be quite low.

In the microarray literature, several authors have suggested *filtering* to reduce the impact that multiple testing adjustment has on detection power (7–12). Conceptually similar screening approaches have also been proposed for variable selection in high-dimensional regression models (13, 14). In filtering for microarray applications, the data are first used to identify and remove a set of genes which seem to generate uninformative signal. Second, formal statistical testing is applied only to genes which pass the filter. An effective filter will enrich for true differential expression while simultaneously reducing the number of hypotheses tested at stage two—making multiple testing adjustment less severe. Such filtering is further motivated by the observation that the set of genes which are not differentially expressed can be partitioned into two groups: (*i*) genes that are not expressed in any of the conditions of the experiment or whose reporters on the array lack sensitivity to detect their expression; and (*ii*) genes that are expressed and detectable, but not differentially expressed across conditions.

This two-stage approach, the use of which need not be restricted to gene expression applications, assesses each variable on the basis of both a filter statistic (*U*^{I}) and a test statistic (*U*^{II}). Both statistics are required to exceed their respective cutoffs. Note, however, that the two-stage approach is not equivalent to standard hypothesis testing based on the joint distribution of the filter and test statistics: the latter uses a joint null distribution to compute type I error rate, while the former only considers the null distribution of the stage-two test statistic.

Some authors specifically recommend using *nonspecific* or *unsupervised* filters which do not make use of sample class labels, and they suggest that nonspecific filtering will not interfere with formal statistical testing (7, 9). Nonspecific filter statistics include, for example, the overall variance and overall mean—computed across all arrays, ignoring class label. Some Affymetrix arrays permit Present/Absent calls for each gene; requiring a minimum fraction of Present calls across all arrays also yields a nonspecific filter (15).

While filtering has the potential to substantially increase the number of discoveries (Fig. 1), its validity has been debated. One criticism is that data-based filtering constitutes a statistical test. Ignoring this fact, and computing and adjusting the remaining *p*-values as if filtering had not taken place, may result in overly optimistic adjusted *p*-values and a true false positive rate which is larger than reported. Clearly, increasing the number of discoveries only implies an increase in statistical power if the additional discoveries are enriched for real differential expression. If, on the other hand, filtering simply increases the false positive rate without our knowledge, matters have been made worse rather than better.

In the remainder of this article, we clarify these issues. We first point out pitfalls that can arise when an inappropriate filter statistic is used. We then show that with an appropriate choice of filter and test statistics, discoveries are increased while type I error control is maintained, thereby producing a genuine increase in detection power.

## Results

### Filtering Increases Discoveries.

We considered a dataset obtained from samples of 79 individuals with B-cell acute lymphoblastic leukemia (ALL), for which mRNA profiles were measured using Affymetrix HG-U95Av2 microarrays (16, 17). The samples fell into two groups: 37 with the BCR/ABL mutation and 42 with no observed cytogenetic abnormalities. The Robust Multichip Average algorithm (RMA) was used to preprocess the microarray data and produce an expression summary for each gene in each sample (18). Instructions for accessing these data, and for reproducing the analyses reported here, are given in *SI Text*.

We considered both overall variance and overall mean as filtering criteria. In both cases, the fraction *θ*∈[0,1] of genes with the lowest overall variance (or mean) were removed by the filter. The special case *θ* = 0 corresponds to no filtering. We then applied a standard *t*-test to those genes which passed the filter.

Fig. 1 *A* and *B* shows *R*, the total number of rejections, as a function of the cutoff on FDR-adjusted *p*-values. A good choice of filter substantially increased the number of null hypotheses rejected. For the overall variance filter and *θ* in (0,0.5), procedures with higher values of *θ* dominated those with lower values over a wide range of adjusted *p*-value cutoffs. The overall mean filter, on the other hand, was less effective, particularly for *θ* > 0.10. In fact, for *θ* > 0.25 the overall mean filter led to substantially *fewer* rejections than a standard unfiltered approach (Fig. 1 *B* and *C*).

This difference between the performance of the two filters is not surprising, and provides an example of how prior knowledge can be incorporated into the analysis via choice of filter. Probes on Affymetrix arrays are known to produce a wide range of fluorescence intensities, even in the absence of target, making overall mean a poor predictor for nonexpression (19).

### Pitfalls: Type I Error Control Is Lost.

In the preceding section, we showed that a well-chosen filter can substantially increase the number of null hypotheses rejected. Of course, increased rejections correspond to increased power only if the false positive rate is still under control. In this section, we present several examples which demonstrate that filtering can, for an inappropriate choice of statistics, lead to loss of such control. In subsequent sections, however, we show how to avoid this problem.

In ref. 8 the authors discuss a filter which requires the fraction of present calls to exceed a threshold in at least one condition. Similar results are obtained by requiring the average expression value to be sufficiently large in at least one condition. Although such filters do not meet the nonspecificity criterion, they have a sensible motivation: genes whose products are absent in some conditions but present in others are typically of biological interest. Fig. 2*A* shows, however, that such a strategy has the potential to adversely affect the false positive rate. The conditional null distribution for test statistics passing the filter is not the same as the unconditional distribution, and under some conditions, it can have much heavier tails. If one nonetheless uses the unconditional null distribution to compute *p*-values, these will be overly optimistic, and excess false positives will result.

Certain nonspecific filters, for which the filter statistic does not depend on sample class labels, can also invalidate type I error control. Consider applying the following procedure to a two-class dataset: ignore class labels but cluster the samples using, for example, *k*-means clustering with *k* = 2; filter based on the absolute value of a gene-level *t*-statistic computed for the two inferred clusters. Test genes which pass the filter with a *t*-statistic computed for the two real classes. If there are genes with strong differential expression, clustering will recover the true class labels with high probability, making the filter and test statistics identical. In effect, this procedure computes gene-level *t*-statistics as usual but only formally tests the most extreme results. If the standard *t*-distribution is used to obtain *p*-values, type I error rate control will clearly be lost.

More realistic nonspecific filters can also detrimentally affect the conditional distribution of the test statistic. The *limma* *t*-statistic () is based on an empirical Bayes approach which models the gene-level error variances with a scaled inverse *χ*^{2} distribution. For many microarray datasets, this distribution provides a good fit (4). In ref. 7, an overall variance filter is combined with the *limma* . Because the within-class variance estimator () and the overall variance are correlated, filtering on overall variance will deplete the set of genes with low (Fig. 2*B*). A scaled inverse *χ*^{2} will then no longer provide a good fit to the data passing the filter, causing the *limma* algorithm to produce a posterior degrees-of-freedom estimate of ∞. This has two consequences: (*i*) gene-level variance estimates will be ignored, leading to an unintended analysis based on fold change only; and (*ii*) the *p*-values will be overly optimistic (Fig. 2*C*). See *SI Text* for details.

### Conditional Control Is Sufficient.

Having shown that a two-stage approach need not maintain control of type I error rates, even when a nonspecific filter is used, we now examine conditions under which control is maintained.

First, observe that with filtering, false positives and rejections in general are only made at stage two. Therefore, type I errors cannot arise from those hypotheses that have been filtered out, since none of these are rejected. Second, observe that the distributions of the test statistics at stage two are *conditional* distributions, since we only consider test statistics corresponding to hypotheses which have passed the filter. (The pitfalls we describe above demonstrate that for some filters, this conditioning can in fact change the null distribution.) Combining these two observations, we see that the overall FWER is given by the conditional probability of a false positive at stage two; and the overall FDR, by the conditional expectation of the ratio of false to total discoveries at stage two. To control these type I error rates, we therefore require a filter that leads to a conditional distribution of the which is consistent with the requirements of the *p*-value computation and multiple testing adjustment procedures. One may, of course, adapt these procedures to accommodate conditioning-induced changes in the null distributions. In the next section, however, we will consider a simpler alternative: the use of filters that leave the distributions of true-null test statistics unchanged. In this case, the same procedures which are appropriate for unfiltered data are still appropriate after conditioning on filter passage.

### Marginal Independence of Filter and Test Statistics.

For gene *i*, the two-stage approach employs two statistics, and , but only compares —for those hypotheses passing the filter—to a null distribution. The unconditional null distribution of is often used for this purpose, but will only produce correct *p*-values if the conditional and unconditional null distributions of are the same. When the null distribution of does not depend on the value of , we call this marginal independence for gene *i*.

Several commonly used pairs of statistics satisfy this marginal independence criterion for true-null hypotheses. Let denote the set of indices for true nulls, and **Y**_{i} = (*Y*_{i1},…,*Y*_{in})^{t}, the data for gene *i*. If *Y*_{i1},…,*Y*_{in} are independent and identically distributed normal for each , then both the overall mean and overall variance filter statistics are marginally independent of the standard two-sample *t*-statistic. If, on the other hand, *Y*_{i1},…,*Y*_{in} are only exchangeable for each , then every permutation-invariant filter statistic—including overall mean and variance, and robust versions of the same—is independent of the Wilcoxon rank sum statistic. ANOVA or the Kruskall-Wallis test permit extension to more than two classes. Proofs are given in *SI Text*.

In summary, the pairs of filter and test statics described above are such that for true-null hypotheses, the conditional marginal distributions of the test statistics after filtering are the same as the unconditional distributions before filtering. As a consequence, the unadjusted stage-two *p*-values will have the correct size for single tests. This is an important and necessary starting point for multiple testing adjustments which attempt to control the experiment-wide type I error rate.

### FWER: Bonferroni and Holm Adjustments.

Independence of and for each means that stage-two *p*-values computed using the unconditional null distribution of will be correct. Furthermore, the marginal independence property can be used to directly understand the impact of using the Bonferroni adjustment (or, by extension, the Holm step-down adjustment) in combination with filtering. The Bonferroni correction would ideally adjust *p*-values with multiplication by the expected number of hypotheses passing the filter (see *SI Text*). In fact, we multiply by the observed value of . Often, the researcher fixes , meaning that the two quantities are equal; even when is random, the ratio of to will be close to 1 with high probability when the number of hypotheses is large.

### FWER: Westfall and Young Adjustment.

The Westfall and Young minP or maxT adjustments (20) typically provide the greatest power among generally applicable methods for FWER control. They take full advantage of correlation among *p*-values (or test statistics), and when all null hypotheses are true, the nominal FWER is exact, not an upper bound. The single-step minP adjusted *p*-values, for example, are given by [1]where denotes {1,…,*m*}, denotes the intersection of all null hypotheses whose index is in *A*, *p*_{i} are the observed *p*-values, and *P*_{i}, the random variables. The step-down minP procedure is even less conservative, adjusting the ordered *p*-values *p*_{I(1)} ≤ *p*_{I(2)} ≤ … ≤ *p*_{I(m)} in a similar but progressively less aggressive fashion. See ref. 6 or 20 for details.

When filtering, the Westfall and Young minP adjustment for those *p*-values passing the filter now becomes [2]The same reasoning used to prove that [**1**] controls the FWER may also be used to show that [**2**] provides conditional control of the FWER, given . Further, we have shown above that conditional control for each implies overall control.

Importantly, the distributions of the minima in [**1**] and [**2**] are rarely known. In practice, they are typically estimated by bootstrapping or by permuting sample labels from the original data. Estimation by sample label permutation is appropriate only when, under *H*_{i}, the *Y*_{i1},…,*Y*_{in} are exchangeable. In the *SI Text* we show that if filtering is based on a permutation-invariant statistic (like the overall variance or overall mean) and if the distributions of the components of true-null **Y**_{i} are exchangeable before filtering, then they are also conditionally exchangeable after filtering. Further, filters which change the correlation structure among the *p*-values but which preserve exchangeability will not adversely affect permutation-based Westfall and Young *p*-value adjustment: permutation is performed after filtering, and thus on data which reflect the conditional correlation structure, as required for estimation of the conditional distribution of the minimum in [**2**].

### FDR Control and the Joint Distribution.

FDR-controlling procedures which adjust *p*-values require, at a minimum, accurate computation of single-test type I error rates. When the unconditional null distribution is used to compute *p*-values after filtering, equivalence of the unconditional and conditional null distributions of *U*^{II} is therefore necessary for FDR control—to ensure that the unadjusted, postfilter *p*-values are in fact true *p*-values. The marginal independence criterion guarantees this equivalence.

Adjustment procedures which make no further requirements on dependence among the *p*-values, such as that of ref. 21, can then be applied directly to the postfilter *p*-values to control the FDR. Less conservative and more widely used adjustments such as refs. 22 and 23, on the other hand, make additional assumptions about the joint distribution of the test statistics. A sufficient condition for the method of ref. 22, for example, is positive regression dependence (PRD) on each element from (21). Filtering can, however, change the correlation structure among the *p*-values for null hypotheses passing the filter, even when the marginal independence criterion is satisfied. It is therefore possible that the conditional dependence structure after filtering is inappropriate for some adjustment procedures, even though the unconditional dependence structure before filtering did not present any problems.

In our experience with microarrays, reasonable filters do not create substantial differences between the unconditional and conditional correlation structure of the *p*-values. Further, the dependence conditions under which the more powerful FDR adjustments have been shown to work are more general than even PRD (23). However, if exploration of the data suggests filter-induced problems with the joint distribution, one can revert to the method of ref. 21; whether the loss of power associated with this more conservative approach is offset by gains due to filtering will then depend on the particulars of the data. Alternatively, if strong correlations are present between the variables, a multivariate analysis strategy that takes these into account more explicitly might be preferable to variable-by-variable testing.

### Filtering and the Weighted FDR.

In ref. 24 the authors describe a weighted *p*-value adjustment procedure which increases detection power for those hypotheses of greatest interest to the researcher. Their original procedure uses a priori weights, but ref. 25 suggests the use of data-derived weights based on the overall variance. Filtering, using overall variance and the *p*-value adjustment of ref. 22, is closely related to this data-based weighted adjustment. The two-stage approach compares the ordered *p*-values which pass the filter to progressively less stringent thresholds. Under the weighted procedure, if weight zero is assigned to hypotheses with low overall variance, and weight is assigned to hypotheses with high overall variance, this set of *p*-values is compared to the exact same set of thresholds. The two-stage and weighted approaches are not, however, identical. The two-stage approach never rejects null hypotheses which have been filtered out. In the weighted approach, on the other hand, a weight of zero leads to a less favorable adjustment to the *p*-value, but the corresponding null hypothesis may still be rejected if the evidence against it is strong. As a consequence, under the weighted approach, zero-weight hypotheses can contribute to the number of false positives and the total number of rejections, and thus to the FDR.

The weighted false discovery rate (WFDR) provides a better analog to two-stage filtering. Let *R*_{i} be an indicator for rejection of *H*_{i}, and for a fixed weight vector **w**, define and *Q*(**w**) = 0 otherwise. Then WFDR(**w**) is defined to be the expected value of *Q*(**w**) (24). Unlike the weighted approach to the FDR, hypotheses assigned weight zero make no contribution to the WFDR. As a consequence, two-stage FDR control using the procedure of ref. 22 is exactly equivalent to weighted WFDR control using the procedure of ref. 24. Further, for fixed **w**, this procedure controls the WFDR under the PRD assumption (26). Data-derived weights **W**, however, are random. If PRD also holds conditionally given **W** (or, equivalently, ), then this procedure controls WFDR(**W**), and by implication the two-stage filtering procedure controls the standard FDR.

### Variance Filtering, Fold Change, and the *t*-Statistic.

Practitioners frequently compute per-variable *p*-values, adjust these for multiple testing, but then only pursue findings for which the adjusted *p*-value is significant *and* the observed fold change exceeds some value relevant for their application. While this approach improves interpretability of results, the effective type I error rate is not obvious.

It turns out that such a strategy is related to two-stage filtering. There is a straightforward relationship linking the overall variance, the difference in within-class means (the logarithm of the fold change), and the standard within-class variance estimator used in the *t*-statistic (see [**S3**] in *SI Text*). As a consequence, filtering on overall variance, or equivalently, on overall standard deviation, induces a lower bound on fold change. This bound’s value increases somewhat as the *p*-value decreases, and Fig. 3 illustrates the effect. For small samples, this increase is negligible; for larger sample sizes, however, it is appreciable. Importantly, the induced log-fold-change bound is a multiple of the threshold used in an overall standard deviation filter.

## Discussion

In the context of variable-by-variable statistical testing, numerous authors have suggested filtering as a means of increasing sensitivity. This suggestion is typically motivated by a general purpose experimental technology which interrogates a large number of targets, a substantial (but unknown) fraction of which are in fact uninformative. In the context of gene expression, one often uses stock arrays that interrogate all known or hypothesized gene products. In a given experiment, however, many genes may not be expressed in any of the samples, or not expressed sufficiently to generate informative signal. Similar situations exist in other application domains. We and other authors have shown that filtering has the potential to increase the number of discoveries. Increasing discoveries, however, is only beneficial if the overall false positive rate can still be correctly controlled or estimated.

In this article we have shown that inappropriate filtering has the potential to adversely affect type I error rate control. This effect can occur in two different ways:

The first, more immediate problem arises from dependence between the filter and test statistics. If the two are not independent under the null hypothesis, but the unconditional distribution of the test statistic is nonetheless used to compute nominal *p*-values, single-test error rates may be underestimated. Multiple testing adjustment procedures rely on correct unadjusted *p*-values; without these, control of the experiment-wide error rate can be lost. We provide one solution—the use of filter and test static pairs which are marginally independent under the null hypothesis—and we give some concrete examples. When the sample size is large enough, the use of an empirical null distribution offers another potential solution, provided that the effects of conditioning can be correctly incorporated. Importantly, the filter and test statistics need not be independent when the null hypothesis is false. Indeed, positive correlation between the two statistics under the alternative hypothesis (Fig. 1*D*) is required if one hopes to increase detection power by filtering.

A second, more subtle, problem may also arise; namely, some commonly used *p*-value adjustments only accommodate a certain degree of dependence among the unadjusted *p*-values. Filtering can affect dependence between *p*-values, even when the marginal independence criterion is satisfied. The relevance of this concern is application dependent, but in our experience, it is not a serious problem for microarray gene expression data. Further, we show above that permutation-based implementations of the FWER-controlling procedure of ref. 20 can be safely combined with permutation-invariant filters. The FDR-controlling procedure of ref. 21 can also be applied without additional restrictions, and less conservative FDR-controlling procedures can be applied as well if their requirements are met conditionally.

In addition to analyzing power and type I error rate, we have also pointed out a relationship between filtering by overall variance and filtering by fold change. This relationship has important implications. If variation among samples is low, effects whose size is not of practical importance can nonetheless achieve statistical significance—when, for example, the numerator of the *t*-statistic is small but the denominator is smaller still. Fig. 3 shows that if the *t*-test is preceded by overall variance filtering, discoveries with small effect size are avoided. The magnitude of the induced lower bound on fold change is not obvious from the variance threshold, so we provide software for making the necessary computations in the *genefilter* package for Bioconductor (27).

Moderated *t*-statistics like the *limma* are also often used to avoid discoveries with small effect sizes. Further, the null distribution for is typically more concentrated than that of the standard *t*-statistic. In many cases, this concentration also produces power gains—gains which may exceed those obtained by the combination of variance filtering and the standard *t*-statistic. Can even greater power gains be obtained by combining filtering and moderation? Perhaps, but Fig. 2*C* shows that such an approach has the potential to inflate the false positive rate when the sample size is small. Thus, we do not recommend combining *limma* with a filtering procedure which interferes with its distributional assumptions. We are therefore left with two options: variance filtering combined with the standard *T*, or an unfiltered . Each option addresses the issue of small effect sizes, and each can improve power. Which one provides the best improvement is data dependent, and we provide further examples and discussion in *SI Text*.

We have pointed out a close relationship between filtering, a weighted approach to FDR, and WFDR control. Filtering is analogous to the use of a common weight () for all hypotheses passing the filter, and weight zero for the remainder. The use of continuously varying weights, on the other hand, has been shown to be optimal for certain experiment-wide definitions of type I error rate and power, and schemes for data-based estimation of these weights have been proposed (28, 29). Our aim in this article, however, has not been to identify an optimal procedure, but rather to better understand filtering and to explore its effect on power and error rate control. Further, the simplicity of filtering—in terms of both implementation and interpretation—is very appealing and may offset a degree of suboptimality.

Finally, Fig. 1 shows that a poor choice of filter statistic or cutoff can actually reduce detection power. Power can be substantially improved, on the other hand, when the filter and cutoff are chosen to leverage prior knowledge about the assay’s behavior and the underlying biology. Because such choices are application specific, data visualization is crucial. Tools which generate diagnostic plots like those of Fig. 1 are provided in the *genefilter* package. In summary, filtering is not just an algorithmic improvement to *p*-value adjustment; instead, when applied appropriately, it is an intuitive way of incorporating additional information, resulting in a better model for the data.

## Acknowledgments

The authors thank Julien Gagneur, Bernd Fischer, and Terry Speed for helpful input and discussion. This research was supported by funding from the European Community’s FP7, Grant HEALTH-F2-2008-201666.

## Footnotes

^{1}To whom correspondence should be addressed. E-mail: whuber{at}embl.de.Author contributions: R.B., R.G., and W.H. designed and performed research; and R.B. and W.H. analyzed data and wrote the paper.

The authors declare no conflict of interest.

This article is a PNAS Direct Submission.

This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.0914005107/-/DCSupplemental.

Freely available online through the PNAS open access option.

## References

- ↵
- ↵
- Lönnstedt I,
- Speed TP

- ↵
- Tusher VG,
- Tibshirani R,
- Chu G

- ↵
- Smyth GK

- ↵
- Robinson MD,
- Smyth GK

- ↵
- Dudoit S,
- Shaffer JP,
- Boldrick JC

- ↵
- Gentleman R,
- Carey VJ,
- Huber W,
- Irizarry RA,
- Dudoit S

- Scholtens D,
- von Heydebreck A

- ↵
- ↵
- Talloen W,
- et al.

- ↵
- ↵
- ↵
- ↵
- ↵
- ↵
- Affymetrix, Inc.

- ↵
- Chiaretti S,
- et al.

- ↵
- Chiaretti S,
- et al.

- ↵
- ↵
- ↵
- Westfall PH,
- Young SS

- ↵
- ↵
- Benjamini Y,
- Hochberg Y

- ↵
- Storey JD,
- Taylor JE,
- Siegmund D

- ↵
- ↵
- ↵
- Kling YE

- ↵
- ↵
- Rubin D,
- Dudoit S,
- van der Laan M

- ↵
- Roeder K,
- Wasserman L

## Citation Manager Formats

### More Articles of This Classification

### Physical Sciences

### Statistics

### Biological Sciences

### Related Content

- No related articles found.

### Cited by...

- GATA2/3-TFAP2A/C transcription factor network couples human pluripotent stem cell differentiation to trophectoderm with repression of pluripotency
- Reduced generation of lung tissue-resident memory T cells during infancy
- Combined OX40L and mTOR blockade controls effector T cell activation while preserving Treg reconstitution after transplant
- Social and physical environments early in development predict DNA methylation of inflammatory genes in young adulthood
- PD-1 regulates KLRG1+ group 2 innate lymphoid cells
- The kinase TPL2 activates ERK and p38 signaling to promote neutrophilic inflammation
- A CEACAM6-High Airway Neutrophil Phenotype and CEACAM6-High Epithelial Cells Are Features of Severe Asthma
- Improving the Identification of Phenotypic Abnormalities and Sexual Dimorphism in Mice When Studying Rare Event Categorical Characteristics
- Mitochondrial-Nuclear Interactions Mediate Sex-Specific Transcriptional Profiles in Drosophila
- Evolutionary assembly patterns of prokaryotic genomes
- Transcriptomic Signature of the SHATTERPROOF2 Expression Domain Reveals the Meristematic Nature of Arabidopsis Gynoecial Medial Domain
- IL-33 amplifies an innate immune response in the degenerating retina
- Inhibition of the kinase ITK in a mouse model of asthma reduces cell death and fails to inhibit the inflammatory response
- Transcriptome analysis of GVHD reveals aurora kinase A as a targetable pathway for disease prevention
- Placental changes caused by food restriction during early pregnancy in mice are reversible
- TH2 and TH17 inflammatory pathways are reciprocally regulated in asthma
- Genomic determinants of coral heat tolerance across latitudes
- Association of the interferon signature metric with serological disease manifestations but not global activity scores in multiple cohorts of patients with SLE
- Negative feedback buffers effects of regulatory variants
- Identification of Minimal Hepatic Encephalopathy in Patients with Cirrhosis Based on White Matter Imaging and Bayesian Data Mining
- Mucosal effects of tenofovir 1% gel
- Regulation of Neuronal Gene Expression and Survival by Basal NMDA Receptor Activity: A Role for Histone Deacetylase 4
- Gene Expression Profiling Reveals Distinct Molecular Signatures Associated With the Rupture of Intracranial Aneurysm
- Identification of the Alternative Sigma Factor SigX Regulon and Its Implications for Pseudomonas aeruginosa Pathogenicity
- Relationship of Mammographic Density and Gene Expression: Analysis of Normal Breast Tissue Surrounding Breast Cancer
- Detecting differential usage of exons from RNA-seq data
- Reply to I Dahlman
- Reply to Talloen et al.: Independent filtering is a generic approach that needs domain specific adaptation
- Filtering data from high-throughput experiments based on measurement reliability