Distribution of mutation effects on the MIC to amoxicillin in mg/L. (A) For each amino acid along the protein, excluding the signal peptide, the average effect of mutations on MIC is presented in the gene box with a color code, and the effect of each individual amino acid change is presented above. The color code corresponds to the color used in B. Gray bars represent amino acid changes reachable through a single mutation that were not recovered in our mutant library. Amino acids considered in the extended active site are associated with a blue bar beneath the gene box. (B) Distribution of mutation effects on the MIC is presented in color bars (n = 990); white bars illustrate the distribution of MIC of the wild-type clones (n = 1,594), in other words the noise in MIC measurement. (C) Representation of the average effect of mutations on MIC for each residue on the 3D structure of the protein.
