Library measurements vs. individual colony and spike-in controls. (A) Protein levels for 141 sequence-verified constructs characterized by at least two flow cytometry measurements plotted against their FlowSeq-estimated protein levels. One construct of 142 is missing because it had insufficient reads in the FlowSeq analysis. (B) RNA levels for 41 constructs as measured in our library plotted against control constructs spiked into a separate library. One construct of 42 is missing because it had no reads in the spike-in data. (C) Protein levels for 42 control constructs spiked into a separate library plotted against protein levels for those same constructs measured at least twice by flow cytometry. (D) Protein levels for 42 control constructs spiked into a separate library are plotted against protein level measurements as measured in our promoter + RBS library. (All R2 values for linear regressions pass an F test with a P value <2.2e-16.). RFU, relative fluorescent units.
