• Explore more than 100 years of PNAS content!
  • Sign-up for PNAS eTOC Alerts

Composability of regulatory sequences controlling transcription and translation in Escherichia coli

Table 1.

Lookup table of regulatory elements for given RNA and protein levels

Protein levels Low RNA, 0.5 ± 0.13 Medium RNA, 2.1 ± 0.53 High RNA, 6.9 ± 1.73
Low protein: 7,393 ± 1,848 107 P041-R034, P051-R032, P042-R013 69 P084-R002, P070-R006, P061-R040 23 P092-R022, P095-R002, P097-R039
Med protein: 39,450 ± 9,863 95 P055-R032, P017-R107, P022-R096 178 P070-R031, P035-R107, P060-R089 157 P086-R028, P109-R015, P094-R006
High protein: 152,484 ± 38,121 3 P018-R110, P029-R108, P031-R102 252 P055-R055, P049-R090, P056-R086 338 P089-R052, P077-R100, P086-R055
  • We chose three levels of low (17th percentile), medium (50th percentile), and high (83th percentile) RNA and protein levels and determined how many promoter and RBS combinations fall within 25% of those desired levels. The total number of combinations that fall within each range is shown in boldface, along with three examples from each group. RNA levels are given as the measured RNA/DNA ratio, and protein levels are given in relative fluorescence units.

Online Impact

HighWire Press-hosted articles citing this article