SEQUENTIAL TRANSLATION OF TRINUCLEOTIDE CODONS FOR PEPTIDE BOND FORMATION, TRANSLOCATION, AND TERMINATION

  1. Richard K. Tompkins*
  1. NATIONAL HEART AND LUNG INSTITUTE, NATIONAL INSTITUTES OF HEALTH, BETHESDA, MARYLAND

Abstract

The addition of GUA, valyl-tRNA, T factor, GTP, and ethanol to reactions containing fMet-tRNA·AUG·ribosome intermediate results in the synthesis of fMet-valyl-tRNA which can be translocated if G factor is also present. The translocated peptidyl-tRNA can interact with puromycin to form fMet-Val-puromycin. Release of fMet-valine can also take place after translocation if a terminator trinucleotide and its specific release factor are present.

Footnotes

  • ↵ * Present address: Department of Medicine, Dartmouth Medical School, Hanover, N.H. 03755.

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  1. PNAS 1970-08 vol. 66 no. 4 1164-1169
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