PROMITOCHONDRIA OF ANAEROBICALLY GROWN YEAST, IV. CONVERSION INTO RESPIRING MITOCHONDRIA*
Abstract
Promitochondria of anaerobically grown Saccharomyces cerevisiae were selectively labeled in vivo by incubating the cells with [3H]leucine and cycloheximide. When the labeled cells were washed free of cycloheximide and adapted to oxygen in the presence of unlabeled leucine, the respiring mitochondria formed during adaptation proved to be radioactive. In contrast, only insignificant label was found in iso-1-cytochrome c which is synthesized de novo during adaptation. Respiratory adaptation of anaerobically grown yeast thus involves differentiation of promitochondrial organelles.
Footnotes
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↵ † Requests for reprints may be addressed to Dr. G. Schatz, Wing Hall, Cornell University, Ithaca, N.Y. 14850.
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↵ * This study was supported by grants GM 16320 and GM 10422 from the U.S. Public Health Service, a fellowship from the Max Kade Foundation (to H. P.), and a career development award K3-NB-3738 (to M. S.).
