The videos, shown with minimal information and often without sound or music, are meant to provide a sort of scientific cinéma vérité.
Image courtesy of Nipam Patel (University of California, Berkeley, CA).
New Research In
Physical Sciences
Featured Portals
Articles by Topic
Biological Sciences
Featured Portals
Articles by Topic
Abstract
The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends. This novel reaction occurs when the deoxynucleoside at a 5′-end carries a phosphate group and the complementary deoxynucleoside opposite to it carries a 3′-hydroxyl group. The consequence is the lengthening of the original duplex to form dimers or oligomers depending upon whether one or both ends are base-paired.
Studies on Polynucleotides, C A Novel Joining Reaction Catalyzed by the T4-Polynucleotide Ligase
More Articles of This Classification
Related Content
Cited by...
- Bacteriophage T7 DNA Ligase: OVEREXPRESSION, PURIFICATION, CRYSTALLIZATION, AND CHARACTERIZATION
- Rat insulin genes: construction of plasmids containing the coding sequences
- Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites
- DNA Ligase: Structure, Mechanism, and Function
- Deletions in Immunoglobulin Polypeptide Chains as Evidence for Breakage and Repair in DNA
Similar Articles
You May Also be Interested in
Victoria Orphan and Elizabeth Trembath-Reichert discuss microbial life in the deep subseafloor.
PNAS Profile with NAS member and anthropologist Michael Tomasello
Researchers report an early monumental burial site near Lake Turkana in Kenya that may have served as a stable landmark for mobile herders in a changing physical environment and as a social anchor point to foster communal identity and interaction among mobile herders.
A study reports evidence of water ice on the moon’s surface, discerned via a signature in the near-infrared reflectance spectra that suggests the ice was formed by slow condensation due to impact or water migration through the lunar exosphere.
Image courtesy of Pixabay/Ponciano.
