Stimulation of RNA Transcription from Pea and Corn DNA by Protein Retained on Sepharose Coupled to 2,4-Dichlorophenoxyacetic Acid

Abstract

When crude extracts from pea or corn shoots are passed through a column of agarose coupled to a derivative of the synthetic plant hormone 2,4-dichlorophenoxyacetic acid, certain protein fractions are retained, whereas unmodified agarose adsorbs no protein. Appropriate elution yields a protein factor that stimulates DNA-dependent RNA synthesis (supported by Escherichia coli polymerase) by 40-200%. Preliminary evidence suggests that the factor may be influencing initiation of RNA chains.

• E. coli polymerase