Biochemical Differentiation in Reaggregating Brain Cell Culture

  1. Nicholas W. Seeds
  1. Department of Biophysics and Genetics, University of Colorado Medical Center, Denver, Colo. 80220
  2. Department of Psychiatry, University of Colorado Medical Center, Denver, Colo. 80220

Abstract

Dissociated cells from embryonic mouse brain reassociate in rotation culture to form aggregates. During cell culture the specific activities of choline acetyl-transferase (EC 2.3.1.6), acetylcholinesterase (EC 3.1.1.7), and glutamate decarboxylase (EC 4.1.1.15) in the aggregates increase up to twenty-fold, a phenomenon that approximates some of the biochemical events in the development of the mouse brain.

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  1. PNAS August 1, 1971 vol. 68 no. 8 1858-1861
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