Purification of the araC Protein

  1. Gary Wilcox*,
  2. Kenneth J. Clemetson†,
  3. Daniel V. Santi†, and
  4. Ellis Englesberg*
  1. *Department of Biological Sciences, University of California, Santa Barbara, Calif. 93106
  2. †Department of Chemistry, University of California, Santa Barbara, Calif. 93106

Abstract

The araC gene product, a regulatory protein required for expression of the L-arabinose operon, has been purified by affinity chromatography on Sepharose 4B to which 4-aminophenyl-β-D-6-deoxygalactopyranoside (an anti-inducer of the L-arabinose operon) had been covalently attached by means of a 4-aminophenylbutanamido side chain. Evidence is presented showing that the protein binds specifically to ara DNA.

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  1. PNAS September 1, 1971 vol. 68 no. 9 2145-2148
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